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Purified Mouse Anti-Human CD49a
Purified Mouse Anti-Human CD49a
Flow cytometric analysis of CD49A expression on HeLa cell line. HeLa cells were stained with either Purified Mouse Anti-Human CD49a (Cat. No. 559594; solid line histogram) or Purified Mouse IgG1, κ Isotype Control (Cat. No. 555746; dashed line histogram), followed by FITC Goat Anti-Mouse IgG/IgM (Cat. No. 555988). Fluorescent histograms were derived from gated events with the side and forward light-scattering characteristics of viable cells.
Flow cytometric analysis of CD49A expression on HeLa cell line. HeLa cells were stained with either Purified Mouse Anti-Human CD49a (Cat. No. 559594; solid line histogram) or Purified Mouse IgG1, κ Isotype Control (Cat. No. 555746; dashed line histogram), followed by FITC Goat Anti-Mouse IgG/IgM (Cat. No. 555988). Fluorescent histograms were derived from gated events with the side and forward light-scattering characteristics of viable cells.
Product Details
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BD Pharmingen™
Integrin α1; Integrin alpha-1; ITGA1; ITA1; VLA-1; VLA1
Human (QC Testing)
Mouse IgG1, κ
P-Selectin IgG1 Fusion
Flow cytometry (Routinely Tested), Immunohistochemistry-frozen (Tested During Development)
0.5 mg/ml
V S223
AB_397287
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO


Preparation And Storage

Store undiluted at 4°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. An isotype control should be used at the same concentration as the antibody of interest.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Sodium azide is a reversible inhibitor of oxidative metabolism; therefore, antibody preparations containing this preservative agent must not be used in cell cultures nor injected into animals. Sodium azide may be removed by washing stained cells or plate-bound antibody or dialyzing soluble antibody in sodium azide-free buffer. Since endotoxin may also affect the results of functional studies, we recommend the NA/LE (No Azide/Low Endotoxin) antibody format, if available, for in vitro and in vivo use.
  5. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
559594 Rev. 5
Antibody Details
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SR84

The SR84 monoclonal antibody specifically recognizes CD49a (CD49 antigen-like family member A). CD49a is a ~200 kDa type I transmembrane molecule that is encoded by ITGA1 (Integrin subunit alpha 1). CD49a is also known as very late antigen 1α subunit (VLA-1) of the integrin family of cell adhesion molecules. CD49a associates with the integrin β1 subunit (CD29) to form the α1/β1 heterodimer (CD49a/CD29), and serves as receptor for collagen and laminin-1. It is expressed on activated T cells, monocytes, neuronal cells and smooth muscle cells. CD49a has been reported to play a role in cell attachment during the development of both the central and peripheral nervous systems.

559594 Rev. 5
Format Details
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Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
559594 Rev.5
Citations & References
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Development References (4)

  1. Barclay NA, Brown MH, Birkeland ML, et al, ed. The Leukocyte Antigen FactsBook. San Diego, CA: Academic Press; 1997.
  2. Kishimoto T. Tadamitsu Kishimoto .. et al., ed. Leucocyte typing VI : white cell differentiation antigens : proceedings of the sixth international workshop and conference held in Kobe, Japan, 10-14 November 1996. New York: Garland Pub.; 1997.
  3. Rettig WJ, Dracopoli NC, Goetzger TA, et al. Somatic cell genetic analysis of human cell surface antigens: chromosomal assignments and regulation of expression in rodent-human hybrid cells.. Proc Natl Acad Sci USA. 1984; 81(20):6437-41. (Immunogen). View Reference
  4. Schlossman SF. Stuart F. Schlossman .. et al., ed. Leucocyte typing V : white cell differentiation antigens : proceedings of the fifth international workshop and conference held in Boston, USA, 3-7 November, 1993. Oxford: Oxford University Press; 1995.
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559594 Rev. 5

Please refer to Support Documents for Quality Certificates


Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.