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Purified Mouse Anti-Human CD42a
Product Details
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BD Pharmingen™
GPIX; Glycoprotein IX; GP9; Glycoprotein 9
Human (QC Testing)
Mouse IgG1, κ
Flow cytometry (Routinely Tested)
0.5 mg/ml
VI P-34
AB_397127
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO


Preparation And Storage

Store undiluted at 4°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. An isotype control should be used at the same concentration as the antibody of interest.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Sodium azide is a reversible inhibitor of oxidative metabolism; therefore, antibody preparations containing this preservative agent must not be used in cell cultures nor injected into animals. Sodium azide may be removed by washing stained cells or plate-bound antibody or dialyzing soluble antibody in sodium azide-free buffer. Since endotoxin may also affect the results of functional studies, we recommend the NA/LE (No Azide/Low Endotoxin) antibody format, if available, for in vitro and in vivo use.
  5. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
558816 Rev. 4
Antibody Details
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ALMA.16

The ALMA.16 monoclonal antibody specifically recognizes CD42a. CD42a is a 17-22 kDa type I transmembrane glycoprotein that is also known as Platelet glycoprotein IX (GPIX), or Glycoprotein 9 (GP9). CD42a forms a noncovalently linked complex (GPIb/GPIX/GPV) with CD42b, CD42c and CD42d that may serve as a receptor for von Willebrand factor. It is expressed on platelets and megakaryocytes and is absent on the platelets of patients with Bernadr-Soulier Syndrome (BSS). Although the CD42a function is not fully understood, GPIX glycoprotein is important for the assembly and membrane expression of the CD42 complex and for the maintenance of the functional conformation of CD42b (GPIb).

558816 Rev. 4
Format Details
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Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
558816 Rev.4
Citations & References
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Development References (6)

  1. Azorsa DO, Moog S, Cazenave J-P, Lanza F. CD42a–d Workshop: Analysis of antibodies recognizing the platelet GPIb/IX/V (CD42a,b,c,d) complex. In: Kishimoto T. Tadamitsu Kishimoto .. et al., ed. Leucocyte typing VI : white cell differentiation antigens : proceedings of the sixth international workshop and conference held in Kobe, Japan, 10-14 November 1996. New York: Garland Pub.; 1997:651-653.
  2. De Haas M, Von Dem Borne AEGK. CD42a-d Workshop Panel report. In: Kishimoto T. Tadamitsu Kishimoto .. et al., ed. Leucocyte typing VI : white cell differentiation antigens : proceedings of the sixth international workshop and conference held in Kobe, Japan, 10-14 November 1996. New York: Garland Pub.; 1997:648-650.
  3. Fox JE, Aggerbeck LP, Berndt MC. Structure of the glycoprotein Ib.IX complex from platelet membranes. J Biol Chem. 1988; 263(10):4882-4890. (Biology). View Reference
  4. Hickey MJ, Williams SA, Roth GJ. Human platelet glycoprotein IX: an adhesive prototype of leucine-rich glycoproteins with flank-center-flank structures. Proc Natl Acad Sci U S A. 1989; 86(17):6773-6777. (Biology). View Reference
  5. Kishimoto T. Tadamitsu Kishimoto .. et al., ed. Leucocyte typing VI : white cell differentiation antigens : proceedings of the sixth international workshop and conference held in Kobe, Japan, 10-14 November 1996. New York: Garland Pub.; 1997.
  6. Schick PK, Walker J. The acylation of megakaryocyte proteins: glycoprotein IX is primarily myristoylated while glycoprotein Ib is palmitoylated. Blood. 1996; 87(4):1377-1384. (Biology). View Reference
View All (6) View Less
558816 Rev. 4

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Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.