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    PE Mouse Anti-Human P-glycoprotein (CD243)
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    Consider BD Horizon RealYellow™ 586 (RY586) Reagents, a bright and clean fluorochrome alternative to PE off the yellow-green laser. RY586 can be used alongside PE on spectral cytometers. More Info #
    PE Mouse Anti-Human P-glycoprotein (CD243)
    Flow cytometric profile of P-glycoprotein (CD243) expression on PMG-Y cell line. PMG-Y cells were internally transfected and developed to express CD243. They were stained with either PE Mouse IgG2b, ĸ Isotype Control (Cat. No. 555743; dashed line histogram) or PE Mouse Anti-Human P-glycoprotein (CD243) (Cat. No. 557003; solid line histogram). Fluorescent histograms were derived from gated events with the forward and side light-scattering characteristics of viable cells. Flow cytometric analysis was analyzed on a BD FACScan™.
    PE Mouse Anti-Human P-glycoprotein (CD243)
    Flow cytometric profile of P-glycoprotein (CD243) expression on PMG-Y cell line. PMG-Y cells were internally transfected and developed to express CD243. They were stained with either PE Mouse IgG2b, ĸ Isotype Control (Cat. No. 555743; dashed line histogram) or PE Mouse Anti-Human P-glycoprotein (CD243) (Cat. No. 557003; solid line histogram). Fluorescent histograms were derived from gated events with the forward and side light-scattering characteristics of viable cells. Flow cytometric analysis was analyzed on a BD FACScan™.
    Product Details
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    BD Pharmingen™
    CD243; ABCB1; ABC20; CLCS; GP170; MDR1; P-GP; PGY1
    Human (QC Testing)
    Mouse BALB/c IgG2b, κ
    Human MDR1 Transfected Cell Line
    Flow cytometry (Routinely Tested)
    20 µl
    5243
    AB_396549
    Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
    RUO


    Preparation And Storage

    Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed.
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    Product Notices

    1. This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
    2. An isotype control should be used at the same concentration as the antibody of interest.
    3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
    4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
    5. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
    6. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
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    557003 Rev. 9
    Antibody Details
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    17F9

    The 17F9 monoclonal antibody specifically binds to the 170-180 kDa transmembrane glycoprotein (P-glycoprotein), a product of the multidrug resistance-1 (MDR1) gene. This glycoprotein is expressed on MDR positive cells and has been reported to be expressed on many normal tissues, such as adrenal glands and endothelium, in the brain and skin. P-glycoprotein is known to impart drug resistance to cells by pumping many anti-cancer drugs out of the cytoplasm. 17F9 antibody is able to partially block the binding  of UIC2 antibody (another MDR-specific monoclonal antibody).

    557003 Rev. 9
    Format Details
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    PE
    R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
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    PE
    Yellow-Green 488 nm, 532 nm, 561 nm
    496 nm, 566 nm
    576 nm
    557003 Rev.9
    Citations & References
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    Development References (3)

    1. Benard J, Bourhis J, Riou G. Clinical significance of multiple drug resistance in human cancers. Anticancer Res. 1990; 10(5A):1297-1302. (Biology). View Reference
    2. Goldstein LJ, Galski H, Fojo A, et al. Expression of a multidrug resistance gene in human cancers. J Natl Cancer Inst. 1989; 81(2):116-124. (Biology). View Reference
    3. Shi T, Wrin J, Reeder J, Liu D, Ring DB. High-affinity monoclonal antibodies against P-glycoprotein. Clin Immunol Immunopathol. 1995; 76(1):44-51. (Biology). View Reference
    557003 Rev. 9

    Please refer to Support Documents for Quality Certificates


    Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


    Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

    For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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