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    PE Mouse Anti-Human Vγ9 TCR
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    Consider BD Horizon RealYellow™ 586 (RY586) Reagents, a bright and clean fluorochrome alternative to PE off the yellow-green laser. RY586 can be used alongside PE on spectral cytometers. More Info #
    PE Mouse Anti-Human Vγ9 TCR
    Flow cytometric analysis of Vγ9 TCR expression on human peripheral blood lymphocytes. Whole blood was stained with PE Mouse Anti-Human Vγ9 TCR (Cat. No. 555733) and FITC Mouse Anti-Human CD3 (Cat. No. 561807/561806/555332). Erythrocytes were lysed with BD PharmLyse™ Lysing Buffer (Cat. No. 555899). Fluorescent two-color contour plot was derived from gated events with the forward and side light-scattering characteristics of viable lymphocytes. Flow cytometry was performed on a BD FACScan™ system.
    PE Mouse Anti-Human Vγ9 TCR
    Flow cytometric analysis of Vγ9 TCR expression on human peripheral blood lymphocytes. Whole blood was stained with PE Mouse Anti-Human Vγ9 TCR (Cat. No. 555733) and FITC Mouse Anti-Human CD3 (Cat. No. 561807/561806/555332). Erythrocytes were lysed with BD PharmLyse™ Lysing Buffer (Cat. No. 555899). Fluorescent two-color contour plot was derived from gated events with the forward and side light-scattering characteristics of viable lymphocytes. Flow cytometry was performed on a BD FACScan™ system.
    Product Details
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    BD Pharmingen™
    Vgamma9 TCR; V2; TRGC1; TCRGV9; TRGV9
    Human (QC Testing)
    Mouse IgG1, κ
    Flow cytometry (Routinely Tested)
    0.2 mg/ml
    6983
    AB_396076
    Aqueous buffered solution containing ≤0.09% sodium azide.
    RUO


    Preparation And Storage

    Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed.
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    Product Notices

    1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
    2. An isotype control should be used at the same concentration as the antibody of interest.
    3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
    4. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
    5. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
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    555733 Rev. 8
    Antibody Details
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    B3

    The B3 monoclonal antibody specifically recognizes the human variable γ9 subunit (Vγ9) of γ/δ T-cell receptor (TCR). The γ/δ TCR is composed of two disulfide-linked glycoproteins. The gamma chain is approximately 45-60 kD and is detected on a small subset peripheral blood T cells and thymocytes. The majority of normal peripheral blood γ/δ T cells express a Vγ9+/Vδ2+ phenotype. The reason for this selection in the T-cell repertoire is not well understood.

    555733 Rev. 8
    Format Details
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    PE
    R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
    altImg
    PE
    Yellow-Green 488 nm, 532 nm, 561 nm
    496 nm, 566 nm
    576 nm
    555733 Rev.8
    Citations & References
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    Development References (3)

    1. Barclay NA, Brown MH, Birkeland ML, et al, ed. The Leukocyte Antigen FactsBook. San Diego, CA: Academic Press; 1997.
    2. Breit TM, Wolvers-Tettero IL, van Dongen JJ. Receptor diversity of human T-cell receptor gamma delta expressing cells. Prog Histochem Cytochem. 1992; 26(1-4):182-193. (Biology). View Reference
    3. Kabelitz D. Function and specificity of human gamma/delta-positive T cells. Crit Rev Immunol. 1992; 11(5):281-303. (Biology). View Reference
    555733 Rev. 8

    Please refer to Support Documents for Quality Certificates


    Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


    Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

    For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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