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    FITC Mouse Anti-Human CD62P
    FITC Mouse Anti-Human CD62P
    Flow cytometric analysis of CD62P expression on human platelets. Human platelets were either activated with human thrombin (Left Panel) or left resting (Right Panel). The platelets were stained with either FITC Mouse Anti-Human CD62P (Cat. No. 555523/561922) or FITC Mouse IgG1, κ Isotype Control (Cat. No. 555748). The fluorescence histograms were derived from events with the forward and side light-scatter characteristics of platelets, analyzed on a BD FACScan (BDIS, San Jose, CA).
    FITC Mouse Anti-Human CD62P
    Flow cytometric analysis of CD62P expression on human platelets. Human platelets were either activated with human thrombin (Left Panel) or left resting (Right Panel). The platelets were stained with either FITC Mouse Anti-Human CD62P (Cat. No. 555523/561922) or FITC Mouse IgG1, κ Isotype Control (Cat. No. 555748). The fluorescence histograms were derived from events with the forward and side light-scatter characteristics of platelets, analyzed on a BD FACScan (BDIS, San Jose, CA).
    Product Details
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    BD Pharmingen™
    GMP-140; GMP140; LECAM3; P-selectin; PADGEM; PSEL; SELP
    Human (QC Testing)
    Mouse BALB/c IgG1, κ
    Purified Human Platelet Membrane Glycoproteins
    Flow cytometry (Routinely Tested)
    20 µl
    VI P-44
    AB_395909
    Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
    RUO


    Preparation And Storage

    Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with FITC under optimum conditions, and unreacted FITC was removed.
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    Product Notices

    1. This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
    2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
    3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
    4. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
    5. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
    6. An isotype control should be used at the same concentration as the antibody of interest.
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    555523 Rev. 7
    Antibody Details
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    AK-4

    The AK-4 monoclonal antibody specifically binds to CD62P. CD62P is a 140 kDa type I transmembrane glycoprotein that is also known as P-Selectin, Platelet activation-dependent granule membrane protein (PADGEM), or Granule membrane protein-140 (GMP-140). CD62P is expressed by megakaryocytes, activated platelets and activated endothelium. P-Selectin is stored in the α-granules of platelets and the Weibel-Palade bodies of endothelial cells, and is rapidly transported to the plasma membrane upon activation. P-Selectin is thought to mediate the initial adhesive interactions of neutrophils and monocytes with endothelium in inflammatory responses, and of activated platelets to neutrophils and monocytes in hemostasis.

    555523 Rev. 7
    Format Details
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    FITC
    Fluorescein (FITC) is part of the BD blue family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 494-nm and an emission maximum (Em Max) at 518-nm. FITC is designed to be excited by the Blue laser (488-nm) and detected using an optical filter centered near 520 nm (e.g., a 530/30-nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
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    FITC
    Blue 488 nm
    494 nm
    518 nm
    555523 Rev.7
    Citations & References
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    Development References (4)

    1. De Haas M, Von Dem Borne AEG. CD62P Workshop Panel Report. In: Kishimoto T. Tadamitsu Kishimoto .. et al., ed. Leucocyte typing VI : white cell differentiation antigens : proceedings of the sixth international workshop and conference held in Kobe, Japan, 10-14 November 1996. New York: Garland Pub.; 1997:663-664.
    2. Johnson-Tidey RR, McGregor JL, Taylor PR, Poston RN. Increase in the adhesion molecule P-selectin in endothelium overlying atherosclerotic plaques. Coexpression with intercellular adhesion molecule-1. Am J Pathol. 1994; 144(5):952-961. (Biology). View Reference
    3. Kishimoto T. Tadamitsu Kishimoto .. et al., ed. Leucocyte typing VI : white cell differentiation antigens : proceedings of the sixth international workshop and conference held in Kobe, Japan, 10-14 November 1996. New York: Garland Pub.; 1997.
    4. Lasky LA. Selectins: interpreters of cell-specific carbohydrate information during inflammation. Science. 1992; 258(5084):964-969. (Biology). View Reference
    View All (4) View Less
    555523 Rev. 7

    Please refer to Support Documents for Quality Certificates


    Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


    Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

    For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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