FITC Mouse Anti-Mouse I-A[b]

This SKU will be discontinuing Apr 2024. Suggested alternate SKU is [553551] or for additional support, contact your local applications specialist. Contact Us

BD Pharmingen™ FITC Mouse Anti-Mouse I-A[b]

Name: FITC Mouse Anti-Mouse I-A[b]
Brand: BD Pharmingen™
SKU: 553605

Clone 25-9-17

(RUO)

Spectrum Viewer

Protocol Library

Scientific Resources

Product Details

Brand: BD Pharmingen™

Alternative Name: Aβ[b]; H2-IA[b] beta chain; H-2 IA[b] beta chain

Reactivity: Mouse (QC Testing)

Isotype: Mouse C3H, also known as C3H/He, C3H/Bi IgG2a, κ

Immunogen: Mouse C3H.SW Splenocytes

Application: Flow cytometry (Tested During Development)

Concentration: 0.5 mg/ml

Entrez Gene ID: 14961

RRID: AB_394949

Storage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.

Regulatory Status: RUO

Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with FITC under optimum conditions, and unreacted FITC was removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

Recommended Assay Procedures

For immunohistochemical staining (IHC) of cells expressing I-A[b], we recommend the use of biotinylated AF6-120.1 mAb in our special formulation for immunohistochemistry, Cat. No. 550553. For IHC of I-A[d] expressing cells, we recommend biotinylated mAb AMS-32.1, Cat. No. 550554.

Product Notices

Since applications vary, each investigator should titrate the reagent to obtain optimal results.
Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

Companion Products

FITC Mouse IgG2a, κ Isotype Control RUO

Size 0.25 mg Cat No. 553456

553605 Rev. 11

Antibody Details

25-9-17

The 25-9-17 monoclonal antibody specifically recognizes the β chain of the I-A[b] MHC class II alloantigen. It crossreacts with cells from mice of the H-2[d], H-2[p], and H-2[q] haplotypes. Reactivity with other haplotypes (eg, a, f, g7, k, r, s) has not been observed. The strain distribution of the antigen recognized by this reagent is similar or identical to that of anti-I-A[d] (Aβ[d]) mAb 34-5-3.

This antibody is routinely tested by flow cytometric analysis. Other applications were tested at BD Biosciences Pharmingen during antibody development only or reported in the literature.

553605 Rev. 11

Format Details

FITC

Fluorescein (FITC) is part of the BD blue family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 494-nm and an emission maximum (Em Max) at 518-nm. FITC is designed to be excited by the Blue laser (488-nm) and detected using an optical filter centered near 520 nm (e.g., a 530/30-nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.

Format: FITC

Excitation Source: Blue 488 nm

Excitation Max: 494 nm

Emission Max: 518 nm

553605 Rev.11

Citations & References

Development References (4)

Cohn LE, Glimcher LH, Waldmann RA, et al. Identification of functional regions on the I-Ab molecule by site-directed mutagenesis. Proc Natl Acad Sci U S A. 1986; 83(3):747-751. (Clone-specific). View Reference
Ikegami H, Makino S, Harada M, Eisenbarth GS, Hattori M. The cataract Shionogi mouse, a sister strain of the non-obese diabetic mouse: similar class II but different class I gene products. Diabetologia. 1988; 31(4):254-258. (Clone-specific). View Reference
Landias D, Beck BN, Buerstedde JM, et al.. The assignment of chain specificities for anti-Ia monoclonal antibodies using L cell transfectants. J Immunol. 1986; 137(9):3002-3005. (Clone-specific). View Reference
Ozato K, Sachs DH. Monoclonal antibodies to mouse MHC antigens. III. Hybridoma antibodies reacting to antigens of the H-2b haplotype reveal genetic control of isotype expression. J Immunol. 1981; 126(1):317-321. (Immunogen: Cytotoxicity, Flow cytometry). View Reference

View All (4)

553605 Rev. 11

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.