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    Purified Mouse Anti-Human CD124
    Purified Mouse Anti-Human CD124
    Flow cytometric analysis of CD124 expression on human PBMC. Human PBMC isolated by density gradient centrifugation (Ficoll-Paque™) were blocked with normal polyclonal human IgG and stained with either Purified Mouse Anti-Human CD124 (Cat. No. 551894; filled histogram) or Purified Mouse IgG1, κ Isotype Control (Cat. No. 555746; open histogram), followed by Biotin Goat Anti-Mouse Ig (Multiple Adsorption) (Cat. No. 553999) and PE Streptavidin (Cat. No. 554061). Fluorescence histograms depicting CD124 (or Ig isotype) expression were derived from gated events with the forward and side light-scatter characteristics of viable CD19+ lymphocytes.   Note: Certain human cell lines or cell types (e.g., neutrophils and monocytes) can first be treated with reagents that block receptors for the Fc regions of immunoglobulin to avoid nonspecific immunofluorescent staining mediated by Fc receptors.
    Purified Mouse Anti-Human CD124
    Flow cytometric analysis of CD124 expression on human PBMC. Human PBMC isolated by density gradient centrifugation (Ficoll-Paque™) were blocked with normal polyclonal human IgG and stained with either Purified Mouse Anti-Human CD124 (Cat. No. 551894; filled histogram) or Purified Mouse IgG1, κ Isotype Control (Cat. No. 555746; open histogram), followed by Biotin Goat Anti-Mouse Ig (Multiple Adsorption) (Cat. No. 553999) and PE Streptavidin (Cat. No. 554061). Fluorescence histograms depicting CD124 (or Ig isotype) expression were derived from gated events with the forward and side light-scatter characteristics of viable CD19+ lymphocytes.   Note: Certain human cell lines or cell types (e.g., neutrophils and monocytes) can first be treated with reagents that block receptors for the Fc regions of immunoglobulin to avoid nonspecific immunofluorescent staining mediated by Fc receptors.
    Product Details
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    BD Pharmingen™
    IL-4 Receptor α Chain, CD124
    Human (QC Testing)
    Mouse IgG1, κ
    Soluble Human IL-4 Receptor
    Flow cytometry (Routinely Tested)
    0.5 mg/ml
    V C004, BP169; VI BP205, C81
    3566
    AB_394285
    Aqueous buffered solution containing ≤0.09% sodium azide.
    RUO


    Preparation And Storage

    Store undiluted at 4°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
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    Recommended Assay Procedures

    Immunofluorescent Staining and Flow Cytometric Analysis: Purified Mouse Anti-Human CD124 (Cat. No. 551894) antibody can be used for the immunofluorescent staining (≤ 1 µg antibody/10^6 cells) and flow cytometric analysis of human nucleated cells to measure their expressed levels of CD124. An appropriate purified immunoglobulin isotype control is Purified Mouse IgG1, κ Isotype Control (Cat. No. 555746). A three-layer staining protocol is recommended for maximizing the detection of CD124 expressed by cells as detailed in the image legend.

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    Product Notices

    1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
    2. An isotype control should be used at the same concentration as the antibody of interest.
    3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
    4. Ficoll-Paque is a trademark of Amersham Biosciences Limited.
    5. Sodium azide is a reversible inhibitor of oxidative metabolism; therefore, antibody preparations containing this preservative agent must not be used in cell cultures nor injected into animals. Sodium azide may be removed by washing stained cells or plate-bound antibody or dialyzing soluble antibody in sodium azide-free buffer. Since endotoxin may also affect the results of functional studies, we recommend the NA/LE (No Azide/Low Endotoxin) antibody format, if available, for in vitro and in vivo use.
    6. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
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    551894 Rev. 2
    Antibody Details
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    hIL4R-M57

    The hIL4R-M57 antibody specifically binds to the α subunit (IL-4Rα) of the human Interleukin-4 Receptor complex which is also known as CD124. The human IL-4Rα, also known as B cell stimulatory factor 1 receptor (BSF-1 receptor), is a 140 kDa transmembrane glycoprotein that is expressed by B and T lymphocytes and a variety of other hematopoietic and non-hematopoietic cells and cell lines. The cell surface IL-4Rα chain binds IL-4 with high affinity and associates with either the common γ chain (IL-4Rα/γc; aka, type I IL-4R complex) or the IL-13 receptor alpha-1 subunit (IL-4Rα/IL-13Rα1; aka, type II IL-4R complex) to form two distinct types of signal-transducing IL-4R complexes. The type I IL-4 receptor complex specifically binds IL-4 whereas the type II IL-4R complex binds and transduces signals from either IL-4 or IL-13. A truncated form of the IL-4Rα exists in soluble form in biological fluids. In contrast to mice, in humans no distinct mRNA coding for sIL-4R has been described, suggested that human sIL4-R is exclusively produced by proteolytic cleavage of the cell surface receptor. The serum levels of soluble IL-4Rα appear to elevate in pathological situations such as allergy and parasitic infections. Depending on the ratios of IL-4 and sIL-4Rα present in the local milieu, the sIL-4Rα may augment or antagonize the activities of IL-4. The immunogen used to generate the hIL4R-M57 hybridoma was soluble human IL-4R.

    551894 Rev. 2
    Format Details
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    Purified
    Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
    Purified
    551894 Rev.2
    Citations & References
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    Development References (21)

    1. Armitage RJ, Beckmann MP, Idzerda RL, Alpert A, Fanslow WC. Regulation of interleukin 4 receptors on human T cells. Int Immunol. 1990; 2(11):1039-1045. (Biology). View Reference
    2. Armitage RJ, Ziegler SF, Beckmann MP, Idzerda RL, Park LS, Fanslow WC. Expression of receptors for interleukin 4 and interleukin 7 on human T cells. Adv Exp Med Biol. 1991; 292:121-130. (Biology). View Reference
    3. Browning JL, Dougas I, Ngam-ek A, et al. Characterization of surface lymphotoxin forms. Use of specific monoclonal antibodies and soluble receptors.. J Immunol. 1995; 154(1):33-46. (Biology). View Reference
    4. Fanslow WC, Spriggs MK, Rauch CT, et al. Identification of a distinct low-affinity receptor for human interleukin-4 on pre-B cells. Blood. 1993; 81(11):2998-3005. (Biology). View Reference
    5. Gessner A, Rollinghoff M. Biologic functions and signaling of the interleukin-4 receptor complexes. Immunobiology. 2000; 201(3-4):285-307. (Biology). View Reference
    6. Hoffman RC, Castner BJ, Gerhart M, et al. Direct evidence of a heterotrimeric complex of human interleukin-4 with its receptors. Protein Sci. 1995; 4(3):382-386. (Biology). View Reference
    7. Idzerda RL, March CJ, Mosley B, et al. Human interleukin 4 receptor confers biological responsiveness and defines a novel receptor superfamily. J Exp Med. 1990; 171(3):861-873. (Clone-specific). View Reference
    8. Jung T, Bews JP, Enssle KH, Wagner K, Neumann C, Heusser CH. Detection of and discrimination between total and free human interleukin-4 and free soluble interleukin-4 receptor by ELISA. J Immunol Methods. 1998; 217(1-2):41-50. (Biology). View Reference
    9. Jung T, Schrader N, Hellwig M, Enssle KH, Neumann C. Soluble human interleukin-4 receptor is produced by activated T cells under the control of metalloproteinases. Int Arch Allergy Immunol. 1999; 119(1):23-30. (Biology). View Reference
    10. Law CL, Armitage RJ, Villablanca JG, LeBien TW. Expression of interleukin-4 receptors on early human B-lineage cells. Blood. 1991; 78(3):703-710. (Biology). View Reference
    11. Mozo L, Rivas D, Zamorano J, Gutierrez C. Differential expression of IL-4 receptors in human T and B lymphocytes. J Immunol. 1993; 150(10):4261-4269. (Biology). View Reference
    12. Nasert S, Millner M, Enssle KH, Wahn U, Renz H. Differential modulation of T cell functions by soluble IL-4R (s1L-4R) in two cases of severe atopic dermatitis. Pediatr Allergy Immunol. 1996; 7(2):91-94. (Biology). View Reference
    13. Ohara J, Paul WE. Receptors for B-cell stimulatory factor-1 expressed on cells of haematopoietic lineage. Nature. 1987; 325(6104):537-540. (Biology). View Reference
    14. Park LS, Friend D, Sassenfeld HM, Urdal DL. Characterization of the human B cell stimulatory factor 1 receptor. J Exp Med. 1987; 166(2):476-488. (Biology). View Reference
    15. Sang DK, Ouma JH, John CC, et al. Increased levels of soluble interleukin-4 receptor in the sera of patients with visceral leishmaniasis. J Infect Dis. 1999; 179(3):743-746. (Biology). View Reference
    16. Zola H, Flego L, Weedon H. Expression of IL-4 receptor on human T and B lymphocytes. Cell Immunol. 1993; 150(1):149-158. (Clone-specific: Flow cytometry). View Reference
    17. Zola H. Detection of cytokine receptors by flow cytometry. In: Coligan JE, Kruisbeek AM, Margulies DH, Shevach EM, Strober W, ed. Current Protocols in Immunology. New York: Green Publishing Associates and Wiley-Interscience; 1995:6.21.1-6.21.18.
    18. Zola H. Detection of receptors for cytokines and growth factors. The Immunologist. 1994:47.
    19. Zuber CE, Galizzi JP, Harada N, Durand I, Banchereau J. Interleukin-4 receptors on human blood mononuclear cells. Cell Immunol. 1990; 129(2):329-340. (Biology). View Reference
    20. Zuber CE, Galizzi JP, Valle A, Harada N, Howard M, Banchereau J. Interleukin 4 receptors on normal human B lymphocytes: characterization and regulation. Eur J Immunol. 1990; 20(3):551-555. (Biology). View Reference
    21. Zurawski SM, Chomarat P, Djossou O, et al. The primary binding subunit of the human interleukin-4 receptor is also a component of the interleukin-13 receptor. J Biol Chem. 1995; 270(23):13869-13878. (Biology). View Reference
    View All (21) View Less
    551894 Rev. 2

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