Flow cytometric analysis of expression of CD18 on peripheral blood lymphocytes. Whole blood was stained with APC Mouse Anti-Human CD18 (Cat. No. 551060; solid line histogram) or with an APC Mouse IgG1, κ Isotype Control (Cat. No. 555751; dashed line histogram). The erythrocytes were lysed with Lysing Buffer (Cat. No. 555899). The fluorescence histograms were derived from gated events with the forward and side light-scattering characteristics of viable lymphocytes.
Flow cytometric analysis of expression of CD18 on peripheral blood lymphocytes. Whole blood was stained with APC Mouse Anti-Human CD18 (Cat. No. 551060; solid line histogram) or with an APC Mouse IgG1, κ Isotype Control (Cat. No. 555751; dashed line histogram). The erythrocytes were lysed with Lysing Buffer (Cat. No. 555899). The fluorescence histograms were derived from gated events with the forward and side light-scattering characteristics of viable lymphocytes.
Product Details
BD Pharmingen™
ITGB2; Integrin beta-2; Beta subunit for LFA-1, MAC-1/CR3, and p150,95
Human (QC Testing), Rhesus, Cynomolgus, Pig, Rabbit (Tested in Development)
Mouse BALB/c IgG1, κ
Cloned Human DS6 TCR γδ+ Cells
Flow cytometry (Routinely Tested)
20 µl
V BP368
3689
AB_398485
Aqueous buffered solution containing BSA, protein stabilizer, and ≤0.09% sodium azide.
RUO
Preparation And Storage
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to APC under optimum conditions, and unconjugated antibody and free APC were removed.
Product Notices
- This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
- An isotype control should be used at the same concentration as the antibody of interest.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- This APC-conjugated reagent can be used in any flow cytometer equipped with a dye, HeNe, or red diode laser.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
Companion Products
Stain Buffer (BSA) RUO
Size
500 mL
Cat No.
554657
Stain Buffer (FBS) RUO
Size
500 mL
Cat No.
554656
Lysing Solution 10X Concentrate CE_IVD
Size
100 mL
Cat No.
349202
Lysing Buffer RUO
Size
100 mL
Cat No.
555899
APC Mouse IgG1, κ Isotype Control RUO
Size
100 Tests
Cat No.
555751
551060 Rev. 6
Antibody Details
6.7
The 6.7 monoclonal antibody specifically binds to β2 integrin, a 90-95 kDa transmembrane protein, which associates non-covalently as a heterodimer with the a chains of CD11a, CD11b, or CD11c. CD18 is expressed on lymphocytes, monocytes, and more weakly on granulocytes. LFA-1 has been shown to play an important role in homotypic and heterotypic cellular adhesion in immune and inflammatory responses.
551060 Rev. 6
Format Details
APC
Allophycocyanin (APC), is part of the BD family of phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 651 nm and an emission maximum (Em Max) at 660 nm. APC is designed to be excited by the Red (627-640 nm) laser and detected using an optical filter centered near 660 nm (e.g., a 660/20 nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
APC
Red 627-640 nm
651 nm
660 nm
551060 Rev.6
Citations & References
Development References (2)
-
Barclay NA, Brown MH, Birkeland ML, et al, ed. The Leukocyte Antigen FactsBook. San Diego, CA: Academic Press; 1997.
-
Schlossman SF. Stuart F. Schlossman .. et al., ed. Leucocyte typing V : white cell differentiation antigens : proceedings of the fifth international workshop and conference held in Boston, USA, 3-7 November, 1993. Oxford: Oxford University Press; 1995.
551060 Rev. 6
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
