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Purified Mouse Anti-Human CD3
Product Details
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BD™
CD3-epsilon; CD3E; Leu4; T-cell surface antigen T3/Leu-4 epsilon chain; T3E
Human
Mouse BALB/c IgG1, κ
Human Thymocytes
Flow cytometry
6.25 μg/mL
20 μL
II T118; III T492
916
Phosphate buffered saline with gelatin 0.1% sodium azide.
RUO (GMP)


Preparation And Storage

Store vials at 2°C to 8°C. Conjugated forms should not be frozen. Protect from exposure to light. Each reagent is stable until the expiration date shown on the bottle label when stored as directed.

347340 Rev. 1
Antibody Details
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SK7

The CD3 antibody, clone SK7, is derived from the hybridization of NS-1 mouse myeloma cells with spleen cells isolated from BALB/c mice immunized with human thymocytes.

The CD3 antibody reacts with the epsilon chain of the CD3 antigen/TCR complex. This complex is composed of at least six proteins that range in molecular weight from 20 to 30 kilodaltons (kDa). The antigen recognized by CD3 antibodies is noncovalently associated with either α/β or γ/δ TCR (70 to 90 kDa).

347340 Rev. 1
Format Details
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Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
347340 Rev.1
Citations & References
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Development References (21)

  1. Allison JP, Lanier LL. Structure, function, and serology of the T-cell antigen receptor complex. Ann Rev Immunol. 1987; 5:503–540. (Biology).
  2. Brenner M, Groh V, Porcelli A, et al. Knapp W, Dörken B, Gilks W, et al, ed. Leucocyte Typing IV: White Cell Differentiation Antigens. 1989:1049-1053.
  3. Campbell MJ, Scott J, Maecker HT, Park JW, Esserman LJ. Immune dysfunction and micrometastases in women with breast cancer. Breast Cancer Res Treat. 2005; 91:163-171. (Biology).
  4. Centers for Disease Control. Update: universal precautions for prevention of transmission of human immunodeficiency virus, hepatitis B virus, and other bloodborne pathogens in healthcare settings. MMWR. 1988; 37:377-388. (Biology).
  5. Clevers H, Alarcón B, Wileman T, Terhorst C. The T cell receptor/CD3 complex: a dynamic protein ensemble. Annual Rev Immunol. 1988; 6:629. (Biology).
  6. Clinical and Laboratory Standards Institute. 2005. (Biology).
  7. Freidrich W, O'Reilly R, Kozenger B, Gebhard D, Good R, Evans R. T lymphocyte reconstitution in recipients of bone marrow transplants: Analysis of regulatory T-cell imbalances in GVHD. Blood. 1982; 59:696. (Biology).
  8. Garson JA, Beverley PCL, Coakham HB, Harper EJ. Monoclonal antibodies against human T lymphocytes label Purkinje neurones of many species. Nature. 1982; 298:375-377. (Biology).
  9. Haynes BF. Summary of T-cell studies performed during the Second International Workshop and Conference on Human Leukocyte Differentiation Antigens. In: Reinherz EL. Ellis L. Reinherz .. et al., ed. Leukocyte typing II. New York: Springer-Verlag; 1986:3-30.
  10. Kan EAR, Wang CY, Wang LC, Evans RL. Noncovalently bonded subunits of 22 and 28 kd are rapidly internalized by T cells reacted with Anti–Leu-4 antibody. J Immunol. 1983; 131:536-539. (Biology).
  11. Kan EAR, Wang CY, Wang LC, Evans RL. Noncovalently bonded subunits of 22 and 28 kd are rapidly internalized by T cells reacted with Anti–Leu-4 antibody. J Immunol. 1983; 131:536-539. (Biology).
  12. Kaneoka H, Perez-Rojas G, Sasasuki T, Benike CJ, Engleman EG. Human T lymphocyte proliferation induced by a pan-T monoclonal antibody (anti-Leu 4): heterogeneity of response is a function of monocytes. J Immunol. 1983; 131(1):158-164. (Biology). View Reference
  13. Knowles RW. Immunochemical analysis of the T-cell–specific antigens. In: Reinherz EL. Ellis L. Reinherz .. et al., ed. Leukocyte typing II. New York: Springer-Verlag; 1986:259-288.
  14. Kurrle R. Knapp W, ed. Leucocyte Typing IV. New York, NY: Oxford University Press; 1989:290-293.
  15. Lanier LL, Allison JP, Phillips JH. Correlation of cell surface antigen expression on human thymocytes by multi-color flow cytometric analysis: implications for differentiation. J Immunol. 1986; 137(8):2501-2507. (Biology). View Reference
  16. Ledbetter JA, Evans RL, Lipinski M, Cunningham-Rundles C, Good RA, Herzenberg LA. Evolutionary conservation of surface molecules that distinguish T lymphocyte helper/inducer and cytotoxic/suppressor subpopulations in mouse and man. J Exp Med. 1981; 153(2):310-323. (Biology). View Reference
  17. Maecker HT, Maino VC. Analyzing T-cell responses to cytomegalovirus by cytokine flow cytometry. Hum Immunol. 2004; 65:493-499. (Biology).
  18. Maino VC, Picker LJ. Identification of functional subsets by flow cytometry: intracellular detection of cytokine expression.. Cytometry. 1998; 34(5):207-15. (Biology). View Reference
  19. Reichert T, DeBruyere M, Deneys V, et al. Lymphocyte subset reference ranges in adult Caucasians. Clin Immunol Immunopathol. 1991; 60(2):190-208. (Biology). View Reference
  20. Tu W, Chen S, Sharp M, et al. Persistent and selective deficiency of CD4+T cell immunity to cytomegalovirus in immunocompetent young children. J Immunol. 2004; 172:3260-3267. (Biology).
  21. van Dongen JJM, Krissansen GW, Wolvers-Tettero ILM, et al. Cytoplasmic expression of the CD3 antigen as a diagnostic marker for immature T-cell malignancies. Blood. 1988; 71:603-612. (Biology).
View All (21) View Less
347340 Rev. 1

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