BD FACSARIA FUSION

view all
Small particle resolution
Small particle resolution

A tube containing beads in 0.3-µm, 0.4-µm, 0.5-µm, and 1.0-µm sizes was collected on a BD FACSAria Fusion. All three populations were resolved from noise.

CFP-transfected HeLa cells
CFP-transfected HeLa cells

The 445-nm laser, available for the BD FACSAria Fusion, excites Cyan Fluorescent Protein (CFP) more efficiently than the 405-nm laser. In this experiment, the same sample of CFP-transfected HeLa cells was acquired on the BD FACSAria™ III system and excited either by the 445-nm laser or the 405-nm laser. The cells excited by the 445-nm laser showed improved separation compared to those excited by the 405-nm laser.

Experiment run on BD FACSAria III.

Mouse bone marrow side population

Mouse bone marrow side population

Mouse bone marrow cells were stained with Hoechst 33342, c-Kit, Sca-1, and lineage markers, and run on a BD FACSAria III equipped with a 375-nm laser, which also is available for the BD FACSAria Fusion.

The SP fraction (red) was limited to those cells expressing c-Kit+ Sca-1++.

Antibodies used were c-Kit PE, Sca-1 PE-Cy™7, Lineage FITC, CD34 APC, CD45 APC-Cy7, PI, Hoechst Blue, and Hoechst Red.

Experiment run on BD FACSAria III
Cancer cell line side population
Cancer cell line side population

As shown in the dot plot, human HT-29 colon cancer cells were stained with Hoechst 33342 and acquired on a BD FACSAria III equipped with a 375-nm laser, which also is available for the BD FACSAria Fusion.

As a control, side population expression was blocked, as represented in the histogram.

Experiment run on BD FACSAria III

10-color CD4 and CD8 memory and effector cell panel

A 10-color CD4 and CD8 memory and effector cell panel and a regulatory T-cell panel were run on a BD FACSAria Fusion. T-cell subsets are readily resolved.

10-color CD4 and CD8 memory and effector cell panel
Cancer cell line side population

As shown in the dot plot, human HT-29 colon cancer cells were stained with Hoechst 33342 and acquired on a BD FACSAria III equipped with a 375-nm laser, which also is available for the BD FACSAria Fusion.

As a control, side population expression was blocked, as represented in the histogram.

Experiment run on BD FACSAria III



For Research Use Only. Not for use in diagnostic or therapeutic procedures.

APC-Cy7: US patent 5,714,386

Cy™ is a trademark of Amersham Biosciences Corp. Cy dyes are subject to proprietary rights of Amersham Biosciences Corp and Carnegie Mellon University and are made and sold under license from Amersham Biosciences Corp only for research and in vitro diagnostic use. Any other use requires a commercial sublicense from Amersham Biosciences Corp, 800 Centennial Avenue, Piscataway, NJ 08855-1327, USA.