In Vitro Fertilization

Overview

BD Falcon pre-tested plasticware for in vitro fertilization (IVF).

BD Falcon™ IVF products are manufactured in compliance with cGMP regulations and are sterilized by gamma irradiation 10^–6 Sterility Assurance Level (SAL). BD Falcon IVF products are certified sterile, non-pyrogenic, and non-embryotoxic. These pre-tested products save you time and expense in complying with the College of American Pathologists and the American Society for Reproductive Medicine recommended standards for IVF labs.

Features

Non-embryotoxic, non-pyrogenic, and non-cytotoxic
Sterilized by gamma irradiation (10^-6 SAL)
Flat-dish bottoms for distortion-free optics
Packaged in peel-open, medical-style packaging
Tissue culture (TC)-treated for a consistent hydrophilic surface (excludes 50 x 9 mm IVF low-wall dish)

Mouse embryo testing

Each lot of BD Falcon IVF product is tested for embryotoxicity using the 1-cell mouse embryo assay. Mouse embryos are isolated at the 1-cell stage from B6C3F1 females following super ovulation with gonadotropin and mating with B6D2F1 males. BD Falcon IVF products are tested using both direct and indirect test methods. In the direct assay, the embryos are cultured directly in the IVF labware. In the indirect assay, culture media is incubated in the IVF labware for 24 hours at 37°C. The incubated media is then transferred to a culture plate and the 1-cell mouse embryo assay is performed. Products are deemed non-embryotoxic if they support the growth of more than 75% of the embryos to the expanded and/or hatched blastocyst stage.

Minimize contamination and evaporation

To improve manipulation of ova and embryos, BD Biosciences collaborated with embryologists to design an innovative 4-well plate. The unique lid reduces the risk of contamination and minimizes evaporation by providing access to two wells at a time, while the other two remain covered. The wells are numbered, and a large writing patch allows clear sample identification. Plates are packaged in individual peel-open trays for sterile presentation.

Flat, optically clear surfaces

BD Falcon IVF dishes and 4-well plates are manufactured from virgin crystalline polystyrene tested for USP Class VI cytotoxicity. They have flat, optically clear surfaces for optimum manipulation and observation of ova and embryos. The lids were designed for aseptic manipulation and consistent venting to maintain humidification. (except the tight-fit lid of the BD Falcon 50 x 9 mm IVF low-wall dish).

BD Falcon 50 x 9 mm IVF Low-Wall Dish

New BD Falcon 50 x 9 mm IVF Low-Wall Dish

BD Biosciences new non-TC treated BD Falcon 50 x 9 mm IVF low-wall dish is designed for a variety of IVF procedures including the Intracytoplasmic Sperm Injection (ICSI) procedure.

Please note: Individual certificates, containing actual test results, are available for each lot by calling your local BD office after you receive your order.

BD Falcon™ 50 x 9 mm IVF Low-Wall Dish

BD Biosciences new non-TC treated BD Falcon™ 50 x 9 mm IVF low-wall dish is designed for a variety of IVF procedures including the Intracytoplasmic Sperm Injection (ICSI) procedure. This dish has many of the same features as the other BD Falcon IVF products except the dish is non-tissue culture treated (non-wettable) for optimal media droplet consistency. The BD Falcon 50 x 9 mm IVF low-wall dish is also a low wall dish making the micro-manipulations of the procedure performed easier. This product is packaged in individually sealed blister compartments so one dish can be accessed at a time and the remaining ones can be kept protected.