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FITC Mouse Anti-Rat CD4
FITC Mouse Anti-Rat CD4
Two-color flow cytometric analysis of the expression of CD4 on rat splenocytes. Lewis splenocytes were incubated simultaneously with FITC Mouse Anti-Rat CD4 (Cat. No. 554843/561834) and PE Mouse Anti-Rat CD3 (Cat. No. 554833) monoclonal antibodies. The CD3-negative CD4-dim cells are the monocyte/macrophage population. The fluorescence contour plots were derived from gated events based on the light-scattering characteristics of viable splenocytes. Flow cytometry was performed on a BD FACScan™.
Two-color flow cytometric analysis of the expression of CD4 on rat splenocytes. Lewis splenocytes were incubated simultaneously with FITC Mouse Anti-Rat CD4 (Cat. No. 554843/561834) and PE Mouse Anti-Rat CD3 (Cat. No. 554833) monoclonal antibodies. The CD3-negative CD4-dim cells are the monocyte/macrophage population. The fluorescence contour plots were derived from gated events based on the light-scattering characteristics of viable splenocytes. Flow cytometry was performed on a BD FACScan™.
Product Details
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BD Pharmingen™
Cd4; p55; W3/25; T-cell surface antigen T4/Leu-3
Rat (QC Testing)
Mouse BALB/c IgG2a, κ
Rat thymocyte glycoproteins
Flow cytometry (Routinely Tested)
0.5 mg/ml
AB_395551
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO


Preparation And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with FITC under optimum conditions, and unreacted FITC was removed.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. An isotype control should be used at the same concentration as the antibody of interest.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
  5. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
561834 Rev. 2
Antibody Details
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OX-38

The OX-38 antibody specifically recognizes the CD4 antigen on most thymocytes, a subpopulation of mature T lymphocytes (ie, MHC class II-restricted T cells, including most T helper cells), monocytes, macrophages, and some dendritic cells. CD4 is an antigen coreceptor on the T-cell surface which interacts with MHC class II molecules on antigen-presenting cells. It participates in T-cell activation through its association with the T-cell receptor complex and protein tyrosine kinase lck. The OX-38 antibody has been reported to bind to the same epitope of CD4 as that recognized by W3/25 mAb, which is a different epitope than that recognized by OX-35 mAb. In vivo blocking of some cell-mediated immune responses by mAb OX-38 has been reported. Injection of OX-38 mAb induces allograft unresponsiveness in rats, with varying results depending on the rat strain used (high or low responder). Furthermore, in vivo depletion of CD4+ lymphocytes has been reported with this antibody.

561834 Rev. 2
Format Details
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FITC
Fluorescein (FITC) is part of the BD blue family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 494-nm and an emission maximum (Em Max) at 518-nm. FITC is designed to be excited by the Blue laser (488-nm) and detected using an optical filter centered near 520 nm (e.g., a 530/30-nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
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FITC
Blue 488 nm
494 nm
518 nm
561834 Rev.2
Citations & References
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Development References (9)

  1. Arima T, Goss JA, Walp LA, Flye MW. Administration of anti-CD4 monoclonal antibody with intrathymic injection of alloantigen results in rat cardiac allograft tolerance. Surgery. 1995; 118(2):265-273. (Clone-specific: Depletion). View Reference
  2. Bañuls MP, Alvarez A, Ferrero I, Zapata A, Ardavin C. Cell-surface marker analysis of rat thymic dendritic cells. Immunology. 1993; 79(2):298-304. (Biology). View Reference
  3. Bierer BE, Sleckman BP, Ratnofsky SE, Burakoff SJ. The biologic roles of CD2, CD4, and CD8 in T-cell activation. Annu Rev Immunol. 1989; 7:579-599. (Biology). View Reference
  4. Janeway CA Jr. The T cell receptor as a multicomponent signalling machine: CD4/CD8 coreceptors and CD45 in T cell activation. Annu Rev Immunol. 1992; 10:645-674. (Biology). View Reference
  5. Jefferies WA, Green JR, Williams AF. Authentic T helper CD4 (W3/25) antigen on rat peritoneal macrophages. J Exp Med. 1985; 162(1):117-127. (Immunogen: Flow cytometry, Functional assay, Immunoaffinity chromatography, Immunoprecipitation, Inhibition). View Reference
  6. Liu L, Zhang M, Jenkins C, MacPherson GG. Dendritic cell heterogeneity in vivo: two functionally different dendritic cell populations in rat intestinal lymph can be distinguished by CD4 expression. J Immunol. 1998; 161(3):1146-1155. (Biology). View Reference
  7. Stitz L, Sobbe M, Bilzer T. Preventive effects of early anti-CD4 or anti-CD8 treatment on Borna disease in rats. J Virol. 1992; 66(6):3316-3323. (Clone-specific: Blocking). View Reference
  8. Suzuki H, Hara MH, Miyahara T, et al. Microchimerism and graft acceptance: IV. Cardiac allograft acceptance following anti-adhesion molecule antibody therapy. Transplant Proc. 1996; 28(4):2058-2060. (Clone-specific: Blocking). View Reference
  9. Yin D, Fathman CG. Tissue-specific effects of anti-CD4 therapy in induction of allograft unresponsiveness in high and low responder . Transpl Immunol. 1995; 3(3):258-264. (Clone-specific: Blocking). View Reference
View All (9) View Less
561834 Rev. 2

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Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.