APC Mouse anti-Human CD39
Clone TU66 (also known as Tü 66, Tü66) (RUO)
- Brand BD Pharmingen™
- Alternative Name ENTPD1; NTPDase-1; Ecto-ATPase 1; Ecto-ATPDase 1
- Vol. Per Test 20 µl
- Isotype Mouse IgG2b, κ
- Reactivity Human (QC Testing)
Flow cytometry (Routinely Tested)
- Workshop No. IV A54
- Entrez Gene ID 953
- Storage Buffer Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
- Regulatory Status RUO
Regulatory Status Legend
The TU66 monoclonal antibody reacts with human CD39, also known as ectonucleoside triphosphate diphosphohydrolase 1 (ENTPD1), an ectoenzyme that degrades ATP to AMP. It is a member of the ectonucleoside triphosphate dihydrolases (E-NTPDases) family involved in the regulation of extracellular nucleotide catabolism by controlling the extracellular nucleoside triphosphate pool (NTP). CD39 is expressed on a subset of T cells, B cells and dendritic cells, with weak staining of monocytes and granulocytes. Recently, CD39 has been found to be expressed primarily by immune-suppressive Foxp3(+) regulatory T (Treg) cells in both human and mice. In humans, CD39 is restricted to a subset of Foxp3+ regulatory effector/memory-like T cells. In mice, the enzyme is present on most, if not all, CD4+CD25+ cells. CD39 expression is driven by Foxp3 and it is thought that CD39 allows Treg cells to enter inflamed areas where high levels of ATP are present.
Allophycocyanin (APC), is an accessory photosynthetic pigment found in blue-green algae. Its molecular weight is approximately 105 kDa. APC has six phycocyanobilin chromophores per molecule, which makes it a very bright fluorochrome that is highly suitable for flow cytometry applications. Due to nearly identical excitation and emission properties but different spillover characteristics, APC and Alexa Fluor® 647 cannot be used simultaneously
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Preparation and Storage
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
The antibody was conjugated to APC under optimum conditions, and unconjugated antibody and free APC were removed.
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
- This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Please refer to www.bdbiosciences.com/pharmingen/protocols for technical protocols.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- Source of all serum proteins is from USDA inspected abattoirs located in the United States.