V450 Rat Anti-Mouse Foxp3

BD Horizon™ V450 Rat Anti-Mouse Foxp3

Name: V450 Rat Anti-Mouse Foxp3
Brand: BD Horizon™
SKU: 561293

Clone MF23

(RUO)

Flow cytometric analysis of Foxp3 expression in BALB/c mouse splenocytes. Splenocytes from BALB/c mice were fixed and permeabilized using working solutions of Mouse Foxp3 Buffers (Mouse Foxp3 Buffer Set, Cat. No. 560409). The cells were then stained with PE Rat anti-Mouse CD4 (Cat. No. 553048) and BD Horizon™ V450 Rat anti-Mouse FoxP3 antibodies (Cat. No. 561293). The flow cytometric dot plot shows the correlated expression of CD4 versus Foxp3 for gated events with the forward and side light-scattering characteristics of viable lymphocytes. Flow cytometry was performed using a BD™ LSR II Flow Cytometry System.

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Product Details

Brand: BD Horizon™

Alternative Name: Forkhead box P3; Forkhead box protein P3; JM2; Scurfin; Scurfy; Sf

Reactivity: Mouse (QC Testing)

Isotype: Rat IgG2b, κ

Immunogen: Mouse FoxP3 Recombinant Protein

Application: Intracellular staining (flow cytometry) (Routinely Tested)

Concentration: 0.2 mg/ml

RRID: AB_10611728

Storage Buffer: Aqueous buffered solution containing protein stabilizer and ≤0.09% sodium azide.

Regulatory Status: RUO

Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The antibody was conjugated to the dye under optimum conditions and unreacted dye was removed.

Recommended Assay Procedures

BD® CompBeads can be used as surrogates to assess fluorescence spillover (compensation). When fluorochrome conjugated antibodies are bound to BD® CompBeads, they have spectral properties very similar to cells. However, for some fluorochromes there can be small differences in spectral emissions compared to cells, resulting in spillover values that differ when compared to biological controls. It is strongly recommended that when using a reagent for the first time, users compare the spillover on cells and BD® CompBeads to ensure that BD® CompBeads are appropriate for your specific cellular application.

Product Notices

Since applications vary, each investigator should titrate the reagent to obtain optimal results.
Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
BD Horizon V450 has a maximum absorption of 406 nm and maximum emission of 450 nm. Before staining with this reagent, please confirm that your flow cytometer is capable of exciting the fluorochrome and discriminating the resulting fluorescence.
Please refer to http://regdocs.bd.com to access safety data sheets (SDS).
Pacific Blue™ is a trademark of Life Technologies Corporation.

Companion Products

Stain Buffer (FBS) RUO

Size 500 mL Cat No. 554656

Mouse Foxp3 Buffer Set RUO

Size 100 Tests Cat No. 560409

PE Rat Anti-Mouse CD4 RUO

Size 0.1 mg Cat No. 553048

561293 Rev. 2

Antibody Details

MF23

Foxp3 is a 50-55 kDa protein also known as Forkhead box P3, JM2, or IPEX. It is a member of the forkhead or winged helix family of

transcription factors and is specifically expressed by T regulatory (Treg) cells. Foxp3 has been reported to be a key regulatory protein for Treg

cell development and function. Ectopic expression of Foxp3 in conventional T cells is sufficient to induce suppressive activity, repress the

production of cytokines such as IL2 and IFN-γ, and upregulate Treg cell-associated molecules such as CD25, CTLA4 and GITR. It has been

found that the mutation of Foxp3 is responsible for "scurfy" mice. When overexpressed, Foxp3 leads to poor T cell proliferation and

activation

561293 Rev. 2

Format Details

V450

BD Horizon™ V450 Dye is part of the BD Horizon™ violet family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 405-nm and an emission maximum (Em Max) at 450-nm. BD Horizon™ V450, driven by BD innovation, is designed to be excited by the violet laser (405 nm) and detected using an optical filter centered near 450-nm (e.g., a 450/50-nm bandpass filter). The dye can be excited by the UV (355-nm) laser resulting in cross-laser excitation and spillover. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.

Format: V450

Excitation Source: Violet 405 nm

Excitation Max: 405 nm

Emission Max: 450 nm

561293 Rev.2

Citations & References

Development References (3)

Hori S, Nomura T, Sakaguchi S. Control of regulatory T cell development by the transcription factor Foxp3. Science. 2003; 299(5609):1057-1061. (Biology). View Reference
Ono M, Yaguchi H, Ohkura N, et al. Foxp3 controls regulatory T-cell function by interacting with AML1/Runx1. Nature. 2007; 446(7136):685-689. (Biology). View Reference
Zheng Y, Rudensky AY. Foxp3 in control of the regulatory T cell lineage. Nat Immunol. 2007; 8:457-462. (Biology). View Reference

561293 Rev. 2

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.