T cell precursors, derived from hematopoietic stem cells in bone marrow, undergo multiple phases of differentiation in the thymus before maturing into functional T cells.
During this development, precursor T cells transiently or stably express specific molecules including T cell lineage markers such as TCR, CD3, CD4, and CD8. By analyzing expressed patterns of various markers, including cell surface receptors and intracellular signaling molecules, researchers have determined the cellular and molecular mechanisms that enable precursors to develop into mature, functional T cells.
A wide array of applications facilitate these studies—preeminent among them is multicolor flow cytometry. Using panels of directly-conjugated fluorescent antibodies to recognize T cell specific epitopes multicolor flow cytometric analysis allows researchers to interrogate specific target molecule levels expressed by individual cells in various phases of development. Using flow cytometry and assay systems such as BD™ Cytometric Bead Array (CBA) and BD™ Phosflow that measure proteins derived from activated T cell subsets, researchers have gained a detailed view of the cellular pathways and molecular mechanisms that support T cell development.
BD Biosciences also offers products that use complementary technologies such as ELISA, ELISPOT, In Vivo Capture, and intracellular staining assays to support T cell research.
For over 20 years, BD Biosciences has actively supported groundbreaking research in the field, with innovative FACS™ brand flow cytometry systems and high quality BD Pharmingen™ and BD FastImmune™ brand reagents designed to simplify the identification, isolation, and characterization of T cells and their interacting partners.