Purified Mouse Anti-Human CD9
Clone M-L13 (RUO)
- Brand BD Pharmingen™
- Alternative Name CD9 antigen (p24); 5H9; BA2; BTCC-1; DRAP-27; GIG2; MIC3; MRP-1; TSPAN29
- Concentration 0.5 mg/ml
- Isotype Mouse BALB/c IgG1, κ
- Reactivity Human (QC Testing)
Flow cytometry (Routinely Tested)
Western blot, Immunohistochemistry-frozen (Tested During Development)
Immunohistochemistry-paraffin (Not Recommended)
- Immunogen Human C-ALL Cells
- Workshop No. III 617
- Entrez Gene ID 928
- Storage Buffer Aqueous buffered solution containing ≤0.09% sodium azide.
- Regulatory Status RUO
Regulatory Status Legend
The M-L13 monoclonal antibody specifically binds to a 24 kDa type III transmembrane protein that is expressed on platelets, pre-B cells, monocytes, endothelial and epithelial cells. CD9 belongs to a family of membrane proteins called tetraspanins that transverse the membrane four times. CD9 is weakly expressed on resting mature B cells. M-L13 induces platelet aggregation and activation. This antibody is also suitable for staining acetone-fixed, frozen tissue sections.
Suggested Companion Products
Preparation and Storage
Store undiluted at 4°C.
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- An isotype control should be used at the same concentration as the antibody of interest.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
- Sodium azide is a reversible inhibitor of oxidative metabolism; therefore, antibody preparations containing this preservative agent must not be used in cell cultures nor injected into animals. Sodium azide may be removed by washing stained cells or plate-bound antibody or dialyzing soluble antibody in sodium azide-free buffer. Since endotoxin may also affect the results of functional studies, we recommend the NA/LE (No Azide/Low Endotoxin) antibody format, if available, for in vitro and in vivo use.
- Please refer to www.bdbiosciences.com/pharmingen/protocols for technical protocols.
This reagent is effective for indirect immunofluorescence staining of human cells for flow cytometric analysis, western blotting with human platelets lysate at 2-4 µg/ml (in-house data), and immunohistochemistry on frozen tissue (spleen/thymus) sections at 2-10 µg/ml (in-house data). However, we do not recommend using this antibody on the formalin fixed paraffin tissue sections.