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FITC Mouse Anti-Human CD91
Product Details
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BD Pharmingen™
A2MR; APOER; APR; IGFBP3R; TGFBR5; LRPA; LRP1
Human (QC Testing)
Mouse BALB/c IgG1, κ
Purified Human α2 Macroglobulin
Flow cytometry (Routinely Tested)
20 µl
V MA110
AB_393708
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
RUO


Preparation And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with FITC under optimum conditions, and unreacted FITC was removed.

Product Notices

  1. This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
  2. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
550496 Rev. 6
Antibody Details
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A2MR-α2

The A2MR-α2 monoclonal antibody specifically reacts with a 600 kDa, type I membrane single protein, also known as the α2 Macroglobulin (α2M) receptor/low density lipoprotein receptor-related protein 1 (LRP-1). Reported to be an endocytic receptor involved with intracellular signalling, lipid homeostasis, clearance of apoptotic cells, and α2 Macroglobulin mediated clearance of secreted amyloid precursor protein found in Alzheimer patients.  The single chain receptor undergoes cleavage, shortly after synthesis, into the 85 kDa transmembrane β chain that non-covalently binds to the extracellular 500-515 kDa a chain. It has a broad cellular distribution, but in the hematopoietic system it is expressed on monocyte lineage cells.  α2M/LRP-1 mediates endocytosis of a variety of ligands including α2M-proteinase complexes, plasminogen activators in complex with plasminogen activator inhibitor,  or Pseudomonas Exotoxin A. Ligand binding to α2M/LRP-1 is followed by rapid transport of the ligand to lysosomes for degradation.

550496 Rev. 6
Format Details
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FITC
Fluorescein (FITC) is part of the BD blue family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 494-nm and an emission maximum (Em Max) at 518-nm. FITC is designed to be excited by the Blue laser (488-nm) and detected using an optical filter centered near 520 nm (e.g., a 530/30-nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
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FITC
Blue 488 nm
494 nm
518 nm
550496 Rev.6
Citations & References
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Development References (3)

  1. Kishimoto T. Tadamitsu Kishimoto .. et al., ed. Leucocyte typing VI : white cell differentiation antigens : proceedings of the sixth international workshop and conference held in Kobe, Japan, 10-14 November 1996. New York: Garland Pub.; 1997.
  2. Ranganathan S, Hattori H, Kashyap ML. A rapid flow cytometric assay for low-density lipoprotein receptors in human peripheral blood mononuclear cells. J Lab Clin Med. 1994; 125(4):479-486. (Biology). View Reference
  3. Schlossman SF. Stuart F. Schlossman .. et al., ed. Leucocyte typing V : white cell differentiation antigens : proceedings of the fifth international workshop and conference held in Boston, USA, 3-7 November, 1993. Oxford: Oxford University Press; 1995.
550496 Rev. 6

Please refer to Support Documents for Quality Certificates


Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.