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Purified Mouse Anti-Human CD71
Product Details
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BD™
TFR; TFRC; Trfr; TfR1; Transferrin receptor protein 1
Human
Mouse BALB/c IgG2a
Pokeweed–stimulated peripheral blood cells
Flow cytometry
100 μg/mL
20 μL
II L-13
7037
Phosphate buffered saline with gelatin and 0.1% sodium azide.
RUO (GMP)


Preparation And Storage

Store vials at 2°C–8°C. Conjugated forms should not be frozen. Protect from exposure to light. Each reagent is stable until the expiration date shown on the bottle label when stored as directed.

347510 Rev. 1
Antibody Details
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L01.1

The CD71 antibody, clone L01.1, is derived from the hybridization of P3-X63-Ag8.653 mouse myeloma cells with spleen cells isolated from BALB/c mice immunized with blast cells from pokeweed mitogen–stimulated peripheral blood cells.

The CD71 antibody is specific for the human transferrin receptor, with a molecular weight of 190 kilodaltons (kDa), 90-kDa reduced.

347510 Rev. 1
Format Details
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Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
347510 Rev.1
Citations & References
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Development References (9)

  1. Centers for Disease Control. Update: universal precautions for prevention of transmission of human immunodeficiency virus, hepatitis B virus, and other bloodborne pathogens in healthcare settings. MMWR. 1988; 37:377-388. (Biology).
  2. Clinical and Laboratory Standards Institute. 2005. (Biology).
  3. Jefferies WA, Brandon MR, Hunt SV, Williams AF, Gatter KC, Mason DY. Transferrin receptor on endothelium of brain capillaries. Nature. 1998; 312(5990):162-163. (Biology). View Reference
  4. Judd W, Poodry CA, Strominger JL. Novel surface antigens expressed on dividing cells but absent from non-dividing cells. J Exp Med. 1980; 152:1430. (Biology).
  5. Larrick JW, Cresswell P. Modulation of cell surface iron transferrin receptors by cellular density and state of activation.. J Supramol Struct. 1979; 11(4):579-86. (Biology). View Reference
  6. Loken MR, Shah VO, Dattilio KL, Civin CI. Flow cytometric analysis of human bone marrow. II. Normal B lymphocyte development. Blood. 1987; 70(5):1316-1324. (Biology). View Reference
  7. Newman R, Schneider C, Sutherland R, Vodinelish L, Greaves M. The transferrin receptor. Trends Biochem Sci. 1982; 1:397. (Biology).
  8. Phillips JH, Le AM, Lanier LL. Natural killer cells activated in a human mixed lymphocyte response culture identified by expression of Leu-11 and class II histocompatibility antigens.. J Exp Med. 1984; 159(4):993-1008. (Biology). View Reference
  9. Schwarting R, Stein H. Knapp W, Dörken B, Gilks WR, et al, ed. Leucocyte Typing IV: White Cell Differentiation Antigens. New York, NY: Oxford University Press; 1989:455-460.
View All (9) View Less
347510 Rev. 1

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