Skip to main content Skip to navigation
Hamburger Menu
Search icon
Country Selector Country Selector
Middle East / Africa (English)
Products

Solutions

Discover & Learn

Resources & Tools

Support

Search icon Search icon
Close Menu
    Alert Icon
    Purified Mouse Anti-Human Trop-2
    Purified Mouse Anti-Human Trop-2
    Flow cytometric analysis of Trop-2.  MCF7 cells (Human breast adenocarcinoma; ATCC HTB-22) were incubated with 0.25 µg of the Purified Mouse Anti-Human Trop-2 antibody (clone 162-46; shaded) or with a mouse IgG1 isotype control (Cat. No. 555746; unshaded) in BD Pharmingen™ Stain Buffer (Cat. No.554656) for 1 hr on ice. Cells were washed twice in Stain Buffer and incubated with 1 µg of a FITC Goat Anti-Mouse Ig secondary antibody (Cat. No.554001) in Stain Buffer for 1 hr on ice. Cells were washed twice and analyzed by flow cytometry.
    Purified Mouse Anti-Human Trop-2
    Flow cytometric analysis of Trop-2.  MCF7 cells (Human breast adenocarcinoma; ATCC HTB-22) were incubated with 0.25 µg of the Purified Mouse Anti-Human Trop-2 antibody (clone 162-46; shaded) or with a mouse IgG1 isotype control (Cat. No. 555746; unshaded) in BD Pharmingen™ Stain Buffer (Cat. No.554656) for 1 hr on ice. Cells were washed twice in Stain Buffer and incubated with 1 µg of a FITC Goat Anti-Mouse Ig secondary antibody (Cat. No.554001) in Stain Buffer for 1 hr on ice. Cells were washed twice and analyzed by flow cytometry.
    Product Details
    Down Arrow Up Arrow


    BD Pharmingen™
    GA733-1; TROP2; EGP-1; EGP1; GP50; M1S1; TACD2
    Human (QC Testing)
    Mouse BALB/c IgG1, κ
    Human BeWo (choriocarcinoma) Cell Line
    Flow cytometry (Routinely Tested), Immunohistochemistry (Reported)
    0.5 mg/ml
    4070
    AB_394150
    Aqueous buffered solution containing ≤0.09% sodium azide.
    RUO


    Preparation And Storage

    Store undiluted at 4°C. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
    Show More blue down arrow Show Less blue up arrow

    Product Notices

    1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
    2. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
    3. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
    Show More blue down arrow Show Less blue up arrow
    551317 Rev. 5
    Antibody Details
    Down Arrow Up Arrow
    162-46

    The 162-46 monoclonal antibody recognizes human Trop-2 which is also known as GA733-1. Trop-2 is encoded by TACSTD2 (tumor-associated calcium signal transducer 2). It is a single-pass type-1 transmembrane glycoprotein. Trop-2 was originally identified to be expressed on human trophoblast and choriocarcinoma cell lines. Trop-2 is expressed in most human carcinomas. Trop-2 appears to be involved in the transduction of an intracellular calcium signal, although the exact physiological mechanism has yet to be elucidated.

    551317 Rev. 5
    Format Details
    Down Arrow Up Arrow
    Purified
    Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
    Purified
    551317 Rev.5
    Citations & References
    Down Arrow Up Arrow

    Development References (4)

    1. Bobos M, Kotoula V, Kaloutsi V, Karayannopoulou G, Papadimitriou CS, Kostopoulos I. Aberrant CCND1 copies and cyclin D1 mRNA expression do not result in the production of functional cyclin D1 protein in anaplastic large cell lymphoma. Histol Histopathol. 2009; 24(8):1035-1048. (Biology: Immunohistochemistry). View Reference
    2. Fornaro M, Dell'Arciprete R, Stella M, et al. Cloning of the gene encoding Trop-2, a cell-surface glycoprotein expressed by human carcinomas. Int J Cancer. 1995; 62(5):610-618. (Biology). View Reference
    3. Lipinski M, Parks DR, Rouse RV, Herzenberg LA. Human trophoblast cell-surface antigens defined by monoclonal antibodies. Proc Natl Acad Sci U S A. 1981; 78(8):5147-5150. (Immunogen). View Reference
    4. Ripani E, Sacchetti A, Corda D, Alberti S. Human Trop-2 is a tumor-associated calcium signal transducer. Int J Cancer. 1998; 76(5):671-676. (Biology). View Reference
    View All (4) View Less
    551317 Rev. 5

    Please refer to Support Documents for Quality Certificates


    Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


    Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

    For Research Use Only. Not for use in diagnostic or therapeutic procedures.

    Website Feedback