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Purified Mouse Anti-CD45
Purified Mouse Anti-CD45
Western blot analysis of CD45 on a Jurkat cell lysate (Human T-cell leukemia; ATCC TIB-152). Lane 1: 1:500, lane 2: 1:1000, lane 3: 1:2000 dilution of the mouse anti-CD45 antibody.
Purified Mouse Anti-CD45
Immunofluorescence staining of Jurkat cells (Human T-cell leukemia; ATCC TIB-152).
Western blot analysis of CD45 on a Jurkat cell lysate (Human T-cell leukemia; ATCC TIB-152). Lane 1: 1:500, lane 2: 1:1000, lane 3: 1:2000 dilution of the mouse anti-CD45 antibody.
Immunofluorescence staining of Jurkat cells (Human T-cell leukemia; ATCC TIB-152).
Product Details
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BD Transduction Laboratories™
Leukocyte Common Antigen; GP180; T200
Human (QC Testing), Mouse, Rat (Tested in Development)
Mouse IgG1
Human CD45 aa. 535-730 (alternatively spliced)
Western blot (Routinely Tested), Immunofluorescence, Immunoprecipitation (Tested During Development), Immunohistochemistry (Not Recommended)
180-220 kDa
250 µg/ml
AB_397661
Aqueous buffered solution containing BSA, glycerol, and ≤0.09% sodium azide.
RUO


Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. Store undiluted at -20°C.

Recommended Assay Procedures

Western blot:  Please refer to http://www.bdbiosciences.com/pharmingen/protocols/Western_Blotting.shtml

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
610266 Rev. 1
Antibody Details
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69/CD45

CD45 (also known as leukocyte common antigen, GP180, or T200) is found on all hematopoietic cells except those of red cell origin. The N-terminal domain is a large glycosylated extracellular region of variable length (390-542 amino acids) derived by the alternative splicing of at least three exons (4, 5, or 6). This variation accounts for the different isoforms of CD45 (180-220 kDa). The remainder of the molecule includes a short transmembrane region followed by a large highly conserved cytoplasmic domain of 702 residues. The CD45 cytoplasmic domain possesses an intrinsic protein tyrosine phosphatase (PTPase) activity. This region contains two PTPase domains (domains I and II) which share 40% homology. Both domains are required for optimal PTPase activity and show significant homology with other receptor-like and cytoplasmic PTPases. CD45 plays a critical role in antigen receptor-induced responses of T and B cells. In B cells, CD45 can exist as part of a larger multisubunit complex composed of several tyrosine phosphorylated proteins, including the Src-family PTK, Lyn, or it can exist as the membrane immunoglobulin-associated MB-1/B29 heterodimer. In T cells, CD45 associates with and dephosphorylates both lck and fyn. In addition, CD45 can associate with membrane proteins involved in T cell activation, such as CD2, Thy-1, TCR, and CD26.

610266 Rev. 1
Format Details
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Purified
Tissue culture supernatant is purified by either protein A/G or affinity purification methods. Both methods yield antibody in solution that is free of most other soluble proteins, lipids, etc. This format provides pure antibody that is suitable for a number of downstream applications including: secondary labeling for flow cytometry or microscopy, ELISA, Western blot, etc.
Purified
610266 Rev.1
Citations & References
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Development References (5)

  1. Biffen M, McMichael-Phillips D, Larson T, Venkitaraman A, Alexander D. The CD45 tyrosine phosphatase regulates specific pools of antigen receptor-associated p59fyn and CD4-associated p56lck tyrosine in human T-cells.. EMBO J. 1994; 13(8):1920-1929. (Biology). View Reference
  2. Phipps DJ, Yousefi S, Branch DR. Increased enzymatic activity of the T-cell antigen receptor-associated fyn protein tyrosine kinase in asymptomatic patients infected with the human immunodeficiency virus. Blood. 1997; 90(9):3603-3612. (Biology: Western blot). View Reference
  3. Sieh M, Bolen JB, Weiss A. CD45 specifically modulates binding of Lck to a phosphopeptide encompassing the negative regulatory tyrosine of Lck. EMBO J. 1994; 12(1):315-321. (Biology). View Reference
  4. Wang Y, Liang L, Esselman WJ. Regulation of the calcium/NF-AT T cell activation pathway by the D2 domain of CD45. J Immunol. 2000; 164(5):2557-2564. (Biology: Western blot). View Reference
  5. Wu L, Fu J, Shen SH. SKAP55 coupled with CD45 positively regulates T-cell receptor-mediated gene transcription. Mol Biol Cell. 2002; 22(8):2673-2686. (Biology: Immunoprecipitation, Western blot). View Reference
View All (5) View Less
610266 Rev. 1

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Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.