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Alexa Fluor® 647 Rat Anti-Mouse Blimp-1
Alexa Fluor® 647 Rat Anti-Mouse Blimp-1
Two color flow cytometric analysis of Blimp-1 expression in activated mouse splenocytes.  BALB/c splenic leucocytes were stimulated for 3 days with lipopolysaccharide and stained with PE Rat Anti-Mouse CD45R/B220 antibody (Cat.No. 553089/553090/561878). The cells were subsequently fixed and permeabilized with the BD Pharmingen™ Transcription Factor Buffer Set (Cat. No. 562574/562725) and stained with either Alexa Fluor® 647 Rat IgG2a, κ Isotype Control (Cat.No. 557690; Left Panel) or Alexa Fluor® 647 Rat Anti-Mouse Blimp-1 antibody (Cat. No. 563643; Right Panel). The two-color flow cytometric contour plots show the correlated expression patterns of Blimp-1 (or Ig Isotype control staining) versus CD45R/B220 for gated events with the forward and side light-scatter characteristics of intact activated splenocytes. Unstimulated splenocytes show a staining pattern similar to Ig Isotype stained cells (data not shown). Flow cytometric analysis was performed using a BD FACSCanto™ II Flow Cytometry System.
Two color flow cytometric analysis of Blimp-1 expression in activated mouse splenocytes.  BALB/c splenic leucocytes were stimulated for 3 days with lipopolysaccharide and stained with PE Rat Anti-Mouse CD45R/B220 antibody (Cat.No. 553089/553090/561878). The cells were subsequently fixed and permeabilized with the BD Pharmingen™ Transcription Factor Buffer Set (Cat. No. 562574/562725) and stained with either Alexa Fluor® 647 Rat IgG2a, κ Isotype Control (Cat.No. 557690; Left Panel) or Alexa Fluor® 647 Rat Anti-Mouse Blimp-1 antibody (Cat. No. 563643; Right Panel). The two-color flow cytometric contour plots show the correlated expression patterns of Blimp-1 (or Ig Isotype control staining) versus CD45R/B220 for gated events with the forward and side light-scatter characteristics of intact activated splenocytes. Unstimulated splenocytes show a staining pattern similar to Ig Isotype stained cells (data not shown). Flow cytometric analysis was performed using a BD FACSCanto™ II Flow Cytometry System.
Product Details
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BD Pharmingen™
Prdm1; PR domain zinc finger protein 1; Blimp1; PRDI-BF1; ZNFPR1A1
Mouse (QC Testing), Human (Lack of Reactivity Confirmed in Development)
Rat IgG2a, κ
Mouse Blimp-1 Recombinant Protein
Intracellular staining (flow cytometry) (Routinely Tested)
0.2 mg/ml
AB_2738342
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO


Preparation And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated to Alexa Fluor® 647 under optimum conditions, and unreacted Alexa Fluor® 647 was removed.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. An isotype control should be used at the same concentration as the antibody of interest.
  3. Alexa Fluor® 647 fluorochrome emission is collected at the same instrument settings as for allophycocyanin (APC).
  4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  5. The Alexa Fluor®, Pacific Blue™, and Cascade Blue® dye antibody conjugates in this product are sold under license from Molecular Probes, Inc. for research use only, excluding use in combination with microarrays, or as analyte specific reagents. The Alexa Fluor® dyes (except for Alexa Fluor® 430), Pacific Blue™ dye, and Cascade Blue® dye are covered by pending and issued patents.
  6. Alexa Fluor® is a registered trademark of Molecular Probes, Inc., Eugene, OR.
  7. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
  8. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
563643 Rev. 1
Antibody Details
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5E7

The 5E7 monoclonal antibody specifically binds to mouse B lymphocyte-induced maturation protein 1 (Blimp-1). Blimp-1 is a 98 kDa zinc finger-containing protein that is encoded by the Prdm1 gene and functions as a transcriptional repressor. Blimp-1 is essential for regulating the terminal differentiation of activated B cells into plasma cells. In this process, Blimp-1 suppresses genes involved in B cell proliferation, such as Myc and Bcl6. It controls other genes, such as Xbp1, that are required for upregulating the plasma cell's protein synthesis machinery which is essential for antibody production and secretion. Blimp-1 is an important regulator in other cell types as well. It functions in the regulation of T cell activation and homeostasis and NK cell maturation. Blimp-1 is expressed in activated T cells and can function to inhibit IL-2 production while enhancing the differentiation and expression of several molecules produced by effector T cells.

563643 Rev. 1
Format Details
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Alexa Fluor™ 647
Alexa Fluor™ 647 Dye is part of the BD red family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 653-nm and an emission maximum (Em Max) at 669-nm. Alexa Fluor 647 is designed to be excited by the Red laser (627-640 nm) and detected using an optical filter centered near 520-nm (e.g., a 660/20 nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
Alexa Fluor™ 647
Red 627-640 nm
653 nm
669 nm
563643 Rev.1
Citations & References
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Development References (9)

  1. Crotty S, Johnston RJ, Schoenberger SP. Effectors and memories: Bcl-6 and Blimp-1 in T and B lymphocyte differentiation. Nat Immunol. 2010; 11(2):114-120. (Biology). View Reference
  2. Huang S. Blimp-1 is the murine homolog of the human transcriptional repressor PRDI-BF1. Cell. 1994; 78(1):9. (Biology). View Reference
  3. Johnston RJ, Poholek AC, DiToro D, et al. Bcl6 and Blimp-1 are reciprocal and antagonistic regulators of T follicular helper cell differentiation.. Science. 2009; 325(5943):1006-10. (Biology). View Reference
  4. Kallies A, Carotta S, Huntington ND, et al. A role for Blimp1 in the transcriptional network controlling natural killer cell maturation. Blood. 2011; 117(6):1869-1879. (Immunogen: Western blot). View Reference
  5. Kallies A, Hasbold J, Tarlinton DM, et al. Plasma cell ontogeny defined by quantitative changes in blimp-1 expression. J Exp Med. 2004; 200(8):967-977. (Biology). View Reference
  6. Kallies A, Hawkins ED, Belz GT, et al. Transcriptional repressor Blimp-1 is essential for T cell homeostasis and self-tolerance. Nat Immunol. 2006; 7(5):466-474. (Biology). View Reference
  7. Kim SJ, Gregersen PK, Diamond B. Regulation of dendritic cell activation by microRNA let-7c and BLIMP1. J Clin Invest. 2013; 123(2):823-833. (Biology). View Reference
  8. Martins GA, Cimmino L, Liao J, Magnusdottir E, Calame K. Blimp-1 directly represses Il2 and the Il2 activator Fos, attenuating T cell proliferation and survival. J Exp Med. 2008; 205(9):1959-1965. (Biology). View Reference
  9. Turner CA Jr, Mack DH, Davis MM. Blimp-1, a novel zinc finger-containing protein that can drive the maturation of B lymphocytes into immunoglobulin-secreting cells. Cell. 1994; 77(2):297-306. (Biology). View Reference
View All (9) View Less
563643 Rev. 1

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Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.