Analysis of Sox2 on human embryonic stem (ES) cells. H9 human ES cells (WiCell, Madison, WI) were harvested, fixed in BD Cytofix™ buffer (Cat. No. 554655), permeabilized with BD Phosflow™ Perm/Wash buffer I (Cat. No. 557885) and stained with matching concentrations of a BD™ Horizon V450 Mouse IgG1, κ isotype control (dashed line, Cat. No. 560373) or BD™ Horizon V450 Mouse Anti-Sox2 monoclonal antibody (solid line). Histograms were derived from gated events based on light scattering characteristics for the H9 cell line. Flow cytometry was performed on a BD LSR™ II flow cytometry system. BD Phosflow™ Perm Buffer III can also be used with this antibody conjugate.
Analysis of Sox2 on human ES cell-derived neural stem cells (NSC). NSC derived from H9 human ES cells (WiCell, Madison, WI) were harvested, fixed in BD Cytofix™ buffer (Cat. No. 554655), permeabilized with BD Phosflow™ Perm/Wash buffer I (Cat. No. 557885) and stained with matching concentrations of BD™ Horizon V450 Mouse IgG1, κ isotype control (dashed line, Cat. No. 560373) or BD™ Horizon V450 Mouse Anti-Sox2 monoclonal antibody (solid line). Histograms were derived from gated events based on light scattering characteristics for the H9-derived NSC. Flow cytometry was performed on a BD LSR™ II flow cytometry system. BD Phosflow™ Perm Buffer III can also be used with this antibody conjugate.
Analysis of Sox2 on human embryonic stem (ES) cells. H9 human ES cells (WiCell, Madison, WI) were harvested, fixed in BD Cytofix™ buffer (Cat. No. 554655), permeabilized with BD Phosflow™ Perm/Wash buffer I (Cat. No. 557885) and stained with matching concentrations of a BD™ Horizon V450 Mouse IgG1, κ isotype control (dashed line, Cat. No. 560373) or BD™ Horizon V450 Mouse Anti-Sox2 monoclonal antibody (solid line). Histograms were derived from gated events based on light scattering characteristics for the H9 cell line. Flow cytometry was performed on a BD LSR™ II flow cytometry system. BD Phosflow™ Perm Buffer III can also be used with this antibody conjugate.
Analysis of Sox2 on human ES cell-derived neural stem cells (NSC). NSC derived from H9 human ES cells (WiCell, Madison, WI) were harvested, fixed in BD Cytofix™ buffer (Cat. No. 554655), permeabilized with BD Phosflow™ Perm/Wash buffer I (Cat. No. 557885) and stained with matching concentrations of BD™ Horizon V450 Mouse IgG1, κ isotype control (dashed line, Cat. No. 560373) or BD™ Horizon V450 Mouse Anti-Sox2 monoclonal antibody (solid line). Histograms were derived from gated events based on light scattering characteristics for the H9-derived NSC. Flow cytometry was performed on a BD LSR™ II flow cytometry system. BD Phosflow™ Perm Buffer III can also be used with this antibody conjugate.
Product Details
BD Horizon™
Human (QC Testing), Mouse (Reactivity Confirmed in Development)
Mouse CD, also known as Charles River SD (outbred) IgG1, κ
Human Sox2 Recombinant Protein
Intracellular staining (flow cytometry) (Routinely Tested)
5 µl
AB_10712763
Aqueous buffered solution containing protein stabilizer and ≤0.09% sodium azide.
RUO
Preparation And Storage
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with BD Horizon™ V450 under optimum conditions, and unreacted BD Horizon™ V450 was removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Product Notices
- This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
- Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
- An isotype control should be used at the same concentration as the antibody of interest.
- BD Horizon V450 has a maximum absorption of 406 nm and maximum emission of 450 nm. Before staining with this reagent, please confirm that your flow cytometer is capable of exciting the fluorochrome and discriminating the resulting fluorescence.
- For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
- Pacific Blue™ is a trademark of Molecular Probes, Inc., Eugene, OR.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Companion Products
Stain Buffer (FBS) RUO
Size
500 mL
Cat No.
554656
Fixation Buffer RUO
Size
100 mL
Cat No.
554655
Perm/Wash Buffer I RUO
Size
125 mL
Cat No.
557885
Perm Buffer III RUO
Size
125 mL
Cat No.
558050
V450 Mouse IgG1, κ Isotype Control RUO
Size
0.1 mg
Cat No.
560373
561610 Rev. 1
Antibody Details
O30-678
The monoclonal antibody O30-678 recognizes the Sox2 transcription factor. Sox2 [SRY (sex determining region Y)-box 2] is a member of the SRY-related HMG-box (SOX) family of transcription factors. Sox2 is required for the maintenance of the undifferentiated state of pluripotent stem cells. Complexes of Sox2 with the homeobox transcription factors Oct3/4 and/or Nanog bind to the promoters of a network of genes that are involved in the maintenance of pluripotency and self renewal in stem cells. Sox2 is also a marker of neural stem cells during embryonic development and in the adult brain. The O30-678 antibody recognizes both human and mouse Sox2 proteins.
The antibody is conjugated to BD Horizon™ V450, which has been developed for use in multicolor flow cytometry experiments and is available exclusively from BD Biosciences. It is excited by the Violet laser Ex max of 406 nm and has an Em Max at 450 nm. Conjugates with BD Horizon™ V450 can be used in place of Pacific Blue™ conjugates.
561610 Rev. 1
Format Details
V450
BD Horizon™ V450 Dye is part of the BD Horizon™ violet family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 405-nm and an emission maximum (Em Max) at 450-nm. BD Horizon™ V450, driven by BD innovation, is designed to be excited by the violet laser (405 nm) and detected using an optical filter centered near 450-nm (e.g., a 450/50-nm bandpass filter). The dye can be excited by the UV (355-nm) laser resulting in cross-laser excitation and spillover. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
V450
Violet 405 nm
405 nm
450 nm
561610 Rev.1
Citations & References
Development References (3)
-
Boyer LA, Lee TI, Cole MF, et al. Core transcriptional regulatory circuitry in human embryonic stem cells. Cell. 2005; 122:947-956. (Biology). View Reference
-
Pan G, Thomson JA. Nanog and transcriptional networks in embryonic stem cell pluripotency. Cell Res. 2007; 17:42-49. (Biology). View Reference
-
Takahashi K, Yamanaka S. Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors. Cell. 2006; 126(4):663-676. (Biology). View Reference
561610 Rev. 1
Please refer to Support Documents for Quality Certificates
Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described
Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
