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PE Mouse Anti-Rat CD45R
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PE Mouse Anti-Rat CD45R
The expression of CD45R on rat B lymphocytes. Lewis bone marrow leukocytes (left panel) and splenocytes (right panel) were simultaneously stained with FITC-conjugated anti-rat IgM G53-238 (Cat. No. 553887) and PE-conjugated HIS24 monoclonal antibodies. Flow cytometry was performed on a BD FACScan™ flow cytometry system.
The expression of CD45R on rat B lymphocytes. Lewis bone marrow leukocytes (left panel) and splenocytes (right panel) were simultaneously stained with FITC-conjugated anti-rat IgM G53-238 (Cat. No. 553887) and PE-conjugated HIS24 monoclonal antibodies. Flow cytometry was performed on a BD FACScan™ flow cytometry system.
Product Details
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BD Pharmingen™
Rat (QC Testing)
Mouse BALB/c IgG2b, κ
Low-Density Cells from AO/G Rat Peyer's Patches
Flow cytometry (Routinely Tested)
0.2 mg/ml
AB_395574
Aqueous buffered solution containing ≤0.09% sodium azide.
RUO


Preparation And Storage

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

Product Notices

  1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  2. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
  3. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
  4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
554881 Rev. 11
Antibody Details
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HIS24

The HIS24 antibody reacts with a developmentally regulated form of CD45 found on most B lymphocytes, including developing B cells in the bone marrow and peripheral B cells, but not plasma cells. The level of expression of this CD45R antigen appears to be an indicator of both maturational stages in the B-cell lineage and of functionally distinct B-cell subsets. The HIS24 mAb, in combination with other markers such as TdT and Ig expression, is effective in the identification of B-cell progenitors. CD45 is a member of the Protein Tyrosine Phosphatase (PTP) family: Its intracellular (COOH-terminal) region contains two PTP catalytic domains, and the extracellular region is highly variable due to alternative splicing of exons 4, 5, and 6 (designated A, B, and C, respectively), plus differing levels of glycosylation. The CD45 isoforms detected in the rat are cell type-, maturation-, and activation state-specific. The CD45 isoforms play complex roles in T-cell and B-cell antigen receptor signal transduction.

554881 Rev. 11
Format Details
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PE
R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
altImg
PE
Yellow-Green 488 nm, 532 nm, 561 nm
496 nm, 566 nm
576 nm
554881 Rev.11
Citations & References
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Development References (7)

  1. Deenen GJ, Hunt SV, Opstelten D. A stathmokinetic study of B lymphocytopoiesis in rat bone marrow: proliferation of cells containing cytoplasmic mu-chains, terminal deoxynucleotidyl transferase and carrying HIS24 antigen. J Immunol. 1987; 139(3):702-710. (Biology). View Reference
  2. Hermans MH, Deenen GJ, De Boer N, Bo W, Kroese FG, Opstelten D. Expression of HIS50 Ag: a rat homologue of mouse heat-stable antigen and human CD24 on B lymphoid cells in the rat. Immunology. 1997; 90(1):14-22. (Biology). View Reference
  3. Johnson P, Maiti A, Ng DHW. CD45: A family of leukocyte-specific cell surface glycoproteins. In: Herzenberg LA, Weir DM, Herzenberg LA, Blackwell C , ed. Weir's Handbook of Experimental Immunology, Vol 2. Cambridge: Blackwell Science; 1997:62.1-62.16.
  4. Kroese FG, Butcher EC, Lalor PA, Stall AM, Herzenberg LA. The rat B cell system: the anatomical localization of flow cytometry-defined B cell subpopulations. Eur J Immunol. 1990; 20(7):1527-1534. (Biology). View Reference
  5. Kroese FG, Opstelten D, Wubbena AS, et al. Monoclonal antibodies to rat B lymphocyte (sub-)populations. Adv Exp Med Biol. 1985; 186:81-89. (Immunogen). View Reference
  6. Kroese FG, Wubbena AS, Opstelten D, et al. B lymphocyte differentiation in the rat: production and characterization of monoclonal antibodies to B lineage-associated antigens. Eur J Immunol. 1987; 17(7):921-928. (Immunogen). View Reference
  7. Opstelten D, Deenen GJ, Rozing J, Hunt SV. B lymphocyte-associated antigens on terminal deoxynucleotidyl transferase-positive cells and pre-B cells in bone marrow of the rat. J Immunol. 1986; 137(1):76-84. (Biology). View Reference
View All (7) View Less
554881 Rev. 11

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