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    FITC Mouse anti-Human CD123
    FITC Mouse anti-Human CD123
    Flow cytometric analysis of FITC anti-human CD123 (IL3-Rα) on cells transfeced with human IL3-Rα cDNA. Cells transfected with human IL3-Rα cDNA were  stained with either FITC anti-CD123(clone 7G3, Cat. No. 558663, solid line) or a FITC mouse IgG2a isotype control (Cat. No. 555573, dashed line) and analyzed by flow cytometry.  Flow cytometry was performed on a BD FACSCalibur™ System and the histograms were derived from the gated events based on light scattering characteristics of viable cells.
    FITC Mouse anti-Human CD123
    Flow cytometric analysis of FITC anti-human CD123 (IL3-Rα) on cells transfeced with human IL3-Rα cDNA. Cells transfected with human IL3-Rα cDNA were  stained with either FITC anti-CD123(clone 7G3, Cat. No. 558663, solid line) or a FITC mouse IgG2a isotype control (Cat. No. 555573, dashed line) and analyzed by flow cytometry.  Flow cytometry was performed on a BD FACSCalibur™ System and the histograms were derived from the gated events based on light scattering characteristics of viable cells.
    Product Details
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    BD Pharmingen™
    IL3RA; IL-3RA; IL-3Rα; IL-3R-alpha; Interleukin-3 receptor subunit alpha
    Human (QC Testing)
    Mouse IgG2a, κ
    Human IL-3Ra-transfected cells
    Flow cytometry (Routinely Tested)
    20 µl
    3563
    AB_1645485
    Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
    RUO


    Preparation And Storage

    Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The antibody was conjugated with FITC under optimum conditions, and unreacted FITC was removed. The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
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    Product Notices

    1. This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-µl experimental sample (a test).
    2. For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
    3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
    4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
    5. An isotype control should be used at the same concentration as the antibody of interest.
    6. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
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    561694 Rev. 2
    Antibody Details
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    7G3

    The 7G3 monoclonal antibody specifically reacts with human CD123, the 70 kD IL-3 receptor α chain (IL-3Rα), which associates with the 120-140 kD β subunit. The β chain is shared with the receptors for interleukins IL-5 and GM-CSF. IL-3Rα is expressed on hematopoietic progenitors and plays an important role in hematopoietic progenitor cell growth and differentiation. This antibody has been reported to block the binding of 125I-IL-3 to high and low affinity IL-3 receptors. In functional experiments, this antibody was found to inhibit acute myeloid leukemia cell proliferation, basophil histamine release, endothelial cell-mediated IL-8 secretion, and neutrophil transmigration. This antibody has been reported to be useful for immunoprecipitation, western blot and immunofluorescent staining for flow cytometry. At the Fifth HLDA Workshop, the human IL-3 receptor was designated CD123.

    561694 Rev. 2
    Format Details
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    FITC
    Fluorescein (FITC) is part of the BD blue family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 494-nm and an emission maximum (Em Max) at 518-nm. FITC is designed to be excited by the Blue laser (488-nm) and detected using an optical filter centered near 520 nm (e.g., a 530/30-nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
    altImg
    FITC
    Blue 488 nm
    494 nm
    518 nm
    561694 Rev.2
    Citations & References
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    Development References (2)

    1. Macardle PJ, Chen Z, Shih CY, et al. Characterization of human leucocytes bearing the IL-3 receptor. Cell Immunol. 1996; 168(1):59-68. (Biology). View Reference
    2. Sun Q, Woodcock JM, Rapoport A, et al. Monoclonal antibody 7G3 recognizes the N-terminal domain of the human interleukin-3 (IL-3) receptor alpha-chain and functions as a specific IL-3 receptor antagonist.. Blood. 1996; 87(1):83-92. (Immunogen: Blocking, Immunoprecipitation, Neutralization, Western blot). View Reference
    561694 Rev. 2

    Please refer to Support Documents for Quality Certificates


    Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described


    Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

    For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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