BD ONEFLOW

Non-standardised Laboratory Processes Have Inherent Potential for Mistakes1,2

Specifically designed to address the challenges of a clinical laboratory during the pre-analytical and analytical phases, BD OneFlow™ solution maximizes laboratory efficiency by reducing manual workload, eliminating the need for antibody pipetting and minimizing the risk for operational mistakes and testing repetition. It simplifies the process of instrument standardization, reducing the need for staff training.


Optimized workflow enables efficiency with ready-to-use BD OneFlow™ reagents

Provided in a ready-to-use, dried, single-test tube format, BD OneFlow antibody reagents allow for direct specimen staining, eliminating the need for antibody pipetting, minimizing operational mistakes and the risk for testing repetition.

The reagent barcode allows for both sample and reagent traceability, increasing patient safety and maximizing process efficiency

Antibody inventory management is simplified by reducing the number of antibody vials to be monitored as well as the antibody waste of expired and/or not frequently used antibodies. Featuring room temperature storage, the BD OneFlow reagents eliminate the need for preserving cold-chain shipment and storage, delivering stable products with extended shelf life.

BD OneFlow Antibody Reagents

The BD OneFlow antibody reagents are designed as pre-configured, single-dose, ready-to-use 8-color reagents, which are provided as a single-test tube format in an optimized dried formulation.

The BD antibody reagents can be stored at room temperature for laboratory convenience.

BD OneFlow ALOT

Lab Efficiency - OneFlow ALOT Image

The BD OneFlow ALOT (Acute Leukemia Orientation Tube) is a pre-configured single-dose, ready-to-use 8-color 8-antibodies reagent. Two tubes, one containing the cytoplasmic markers (C tube) and one containing the surface markers (S tube), provide a single-test.

The BD OneFlow ALOT is intended for flow cytometric immunophenotyping of aberrant immature populations of hematopoietic cells (B-lymphoid, T-lymphoid and non-lymphoid lineage) in peripheral blood and bone marrow, as an aid in diagnosis of acute leukemias.

As screening tube, the BD OneFlow ALOT can guide the need for further analysis in combination with panel(s) specifically designed for the classification of different form of B, T and myeloid acute leukemias.

The BD OneFlow ALOT is available in the 10 test/box size (4 pouches of 5 tubes each: 2 pouches of S tubes and 2 pouches of C tubes). Dark red color-coded boxes, pouches and tubes allow for easy visual identification.

The reagent composition is shown below.

Antibody Fluorochrome Clone Tube Target Populations
MPO FITC MPO-7 C Myeloid lineage marker
CD79a PE HM57 C B-lineage marker
CD34 PerCP-Cy™5.5 8G12 S Backbne marker (B-ALL and AML panels).
Identification of immature cells
CD19 PE-Cy™7 SJ25-C1 S Backbone marker (BCP-ALL panel).
B-lineage marker
CD7 APC M-T701 S T-lineage marker
CD3 APC-H7 SK7 S Backbone marker (T-ALL panel).
CD3 Horizon™ V450 UCHT-1 C Backbone marker (T-ALL panel).
Maturity marker for T-cells.
CD45 Horizon™ V500-C 2D1 S Backbone marker.
Identification of immature cells.

For more information, please refer to the Instructions for Use available in 24 languages.

BD OneFlow LST

Lab Efficiency - OneFlow LST Image (no tubes)

The BD OneFlow LST (Lymphoid Screening Tube) is a pre-configured single-dose, ready-to-use 8-color 12-antibodies reagent, that is provided as a single-test tube format.

The BD OneFlow LST is intended for flow cytometric immunophenotyping of normal and aberrant mature lymphocyte populations of B, T and NK cell lineages in peripheral blood, bone marrow, and lymph nodes, as an aid in diagnosis of haematological disorders.

As screening tube, the BD OneFlow LST can guide the need for further analysis in combination with panel(s) specifically designed for the classification of different form of malignancies (B, T or NK). The BD OneFlow LST is available in the 20 test/box size (4 pouches of 5 tubes each).

Dark blue color-coded boxes, pouches and tubes allow for easy visual identification.

Antibody Fluorochrome Clone Target Populations
CD45 BD Horizon™ V500-C 2D1 (anti-HLe-1) Mature lymphocytes, B-cell precursor
CD19 PE-Cy™ 7 SJ25-C1 B cells, T- and NK-cells by exclusion
CD20 BD Horizon™ V450 L27 B cells, T- and NK-cells by exclusion
Anti-Lambda FITC 1-155-2 Normal and clonally expanded B cells
Anti-Kappa PE TB28-2 Normal and clonally expanded B cells
CD38 APC-H7 HB7 Plasma cells and B-cell precursors,
Lymphoid malignancies, NK cells
CD3 APC SK7 T cells, B- and NK-cells by exclusion
CD4 BD Horizon™ V450 SK3 (Leu-3a) T cell subpopulations
CD8 FITC SK1 (Leu-2a) T cell subpopulations
CD5 PerCP-Cy™ 5.5 L17F12 T cell subpopulations
Anti-TCRγδ PE-Cy™ 7 11F2 T cell subpopulations
CD56 PE MY31 (Leu-19) NK cells

For more information, please refer to the Instructions for Use available in 24 languages.

BD OneFlow B-CLPD T1

Lab Efficiency Oneflow B-CLPDT1 Image

The BD OneFlow™ B-CLPD T1 (B-cell Chronic Lymphoproliferative Diseases Tube 1) is a pre-configured single-dose, ready-to-use 8-color reagent that is provided as a single-test tube format.

The BD OneFlow B-CLPD T1 tube is a classification tube that is used for specimens with B-lineage populations needing further investigation in combination with the BD OneFlow LST (Lymphoid Screening Tube). The BD OneFlow B-CLPD T1 is intended for flow-cytometric immunophenotyping of B cells in peripheral blood and bone marrow as an aid in the diagnosis of chronic lymphocytic leukemia (CLL) and other B-cell chronic lymphoproliferative diseases.

It is available in the 20 test/box size (4 pouches of 5 tubes each).

Boxes, pouches and tubes are color coded with a lighter blue color than the one identifying BD OneFlow LST, allowing for reagent visual identification.

The blue color (dark and light) identifies the BD OneFlow B-cell Chronic Lymphoproliferative Disease Panel.

Antibody Fluorochrome Clone Target Populations
CD23 FITC EBVCS-5 Contributes to the classification of CLL or all other mature B-cell diseases
CD10 PE MEM-78 Contributes to the classification of CLL or all other mature B-cell diseases
CD79b PerCP-Cy™5.5 SN8 Contributes to the classification of CLL or all other mature B-cell diseases
CD19 PE-CY™7 SJ25-C1 Backbone marker. In common with BD OneFlow LST
CD200 APC MRC OX-104 Contributes to the classification of CLL or all other mature B-cell diseases
CD43 APC-H7 1G10 Contributes to the classification of CLL or all other mature B-cell diseases
CD20 BD Horizon™ V450 L27 Backbone marker. In common with BD OneFlow LST
CD45 BD Horizon™ V500-C 2D1 Backbone marker. In common with BD OneFlow LST

For more information, please refer to the Instructions for Use available in 24 languages.

BD OneFlow Plasma Cell Disorders (PCD) Panel

The BD OneFlow Plasma Cell Disorders panel comprises two 8-color tubes, 12-markers in total. Both reagents – BD OneFlow PCST and BD OneFlow PCD – are available in the 10 test/box size.

BD OneFlow PCST

Lab Efficiency - OneFlow PCST Image

The BD OneFlow™ PCST (Plasma Cell Screening Tube) is a pre-configured single-dose 8-color reagent, made of two tubes: one containing the cytoplasmic markers (C tube) and one containing the surface markers (S tube).

The BD OneFlow PCST is intended for flow-cytometric immunophenotyping of normal polyclonal and aberrant plasma cell populations in bone marrow as an aid in the diagnosis of hematological disorders.

It is available in the 10 test/box size (4 pouches of 5 tubes each: 2 pouches of S tubes and 2 pouches of C tubes).

Dark green color-coded boxes, pouches and tubes allow for easy visual identification.

Antibody Fluorochrome Clone Tube Target Populations
CD38 FITC HB7 S Backbone marker. Identification of normal and aberrant plasma cells
CD56 PE MY31 S Identification of normal and aberrant plasma cells
β2-Microglobulin PerCP-Cy™5.5 TÜ99 S Prognostic marker
CD19 PE-Cy™7 SJ25-C1 S Backbone marker. Identification of normal and aberrant plasma cells
Anti-Kappa APC TB28-2 C Plasma cells clonality
Anti-Lambda APC-H7 1-155-2 C Plasma cells clonality
CD45 Horizon™ V450 2D1 S Backbone marker. Identification of normal and aberrant plasma cells
CD138 Horizon™ V500-C MI15 S Backbone marker. Identification of plasma cells

For more information, please refer to the Instructions for Use available in 24 languages

BD OneFlow PCD

Lab Efficiency - OneFlow PCD Image

The BD OneFlow™ PCD (Plasma Cell Dyscrasia) tube is a pre-configured single-dose, ready-to-use 8-color reagent.

The BD OneFlow™ PCD tube is a classification tube that shall be used for specimens with plasma cell populations needing further investigation as determined by the BD OneFlow™ PCST (Plasma Cell Screening Tube). The BD OneFlow PCD tube is intended for flow-cytometric immunophenotyping of normal and aberrant plasma cells in bone marrow as an aid in the diagnosis of multiple myeloma or other plasma cell disorders.

It is available in the 10 test/box size (4 pouches of 5 tubes each).

Boxes, pouches and tubes are color coded with a lighter green color than the one identifying BD OneFlow PCST, allowing for reagent visual identification.

The green color (dark and light) identifies the BD OneFlow Plasma Cell Disorder (PCD) Panel.

Antibody Fluorochrome Clone Target Populations
CD38 FITC HB7 Backbone marker. Identification of normal and aberrant plasma cells
CD28 PE L293 Aberrant plasma cells
CD27 PerCP-Cy™5.5 L128 Aberrant plasma cells
CD19 PE-Cy™7 SJ25-C1 Backbone marker. Identification of normal and aberrant plasma cells
CD117 APC 104D2 Aberrant plasma cells
CD81 APC-H7 JS81 Aberrant plasma cells
CD45 Horizon™ V450 2D1 Backbone marker. Identification of normal and aberrant plasma cells
CD138 Horizon™ V500-C MI15 Backbone marker. Identification of plasma cells

For more information, please refer to the Instructions for Use available in 24 languages

Lab efficiency with standardized instrument setup

Specifically designed for BD OneFlow assays, the BD OneFlow assay setup increases efficiency by simplifying instrument standardization, drastically reducing time to results as well as the technical burden and training needs.

BD OneFlow™ Setup Beads

Lab Efficiency - OneFlow Setup Beads

The BD OneFlow Setup Beads guarantee data reproducibility and allow for intra- and inter-laboratory instrument standardization by providing assay-specific target values as per EuroFlow SOPs.4

In association with BD FACSDiva™ CS&T IVD beads and Application Setting module, BD OneFlow Setup Beads deliver daily standardization control and instrument performance monitoring .

BD™ FC Beads - 8-color kit for BD OneFlow™ Assays

Lab Efficiency - OneFlow FC Beads Image

With a simplified procedure for standardizing 8-color compensation, BD™ FC Beads drastically increase laboratory efficiency by minimizing training needs. Available as ready-to-use 3-μm polystyrene beads coupled to fluorochromes and dried in single-use 12 x 75-mm tubes, BD FC Beads eliminate the need for using single-vial reagents as well as label-specific compensation, minimizing the process time (10 minutes for a full 8-color compensation) and reducing the overall cost of operations.


References

  1. Hammerling JA. A Review of Medical Errors in Laboratory Diagnostics and Where We Are Today. Lab Med. 2012;43(2):41–44.
  2. Plebani M, Carraro P. Mistakes in a Stat Laboratory: Types and Frequency. Clin Chem. 1997;43(8):1348–1351.
  3. J.J.M. van Dongen, L. Lhermitte, S. Böttcher, et al on behalf of the EuroFlow Consortium (EU-FP6, LSHB-CT-2006-018708). EuroFlow antibody panels for standardized n-dimensional flow cytometric immunophenotyping of normal, reactive and malignant leukocytes. Leukemia 2012, 26 (9): 1908-75.
  4. T Kalina, J Flores-Montero, VHJ van der Velden, et al on behalf of the EuroFlow Consortium (EUFP6, LSHB-CT-2006-018708). EuroFlow standardization of flow cytometer instrument settings and immunophenotyping protocols; Leukemia 2012, 26 (9): 1986-2010.


Cy™ is a trademark of GE Healthcare.
Cy™ dyes are subject to proprietary rights of GE Healthcare and Carnegie Mellon University and are made and sold under license from GE Healthcare only for research and in vitro diagnostic use. Any other use requires a commercial sublicense from GE Healthcare Corp, 800 Centennial Avenue, Piscataway, NJ 08855-1327, USA.