See What You've Been Missing
The BD FACSCelesta can rapidly and accurately analyze many kinds of cells, including lymphoid tissue (thymus, spleen, and lymph nodes), digested solid tissues, and blood.
Using panels of directly conjugated fluorescent antibodies to recognize specific cell surface and intracellular epitopes, multicolor flow cytometric analysis allows researchers to interrogate specific target protein levels expressed by individual cells in various phases of development and differentiation. The multiparametric, single-cell focus of multicolor flow cytometry is perfectly suited to further immunological discovery of protein and gene expression and cell signaling. BD's solutions for cell identification, transcription factor expression, and cytokine secretion and measurement reflect a commitment to high quality and consistency, which are needed for advanced research.
Illuminate Rare Cells and Low-Density Antigens
Rare cells, or cells that have few surface receptors of a marker of interest, can be difficult to detect using conventional reagents. Bright reagents are essential in resolving these dim cells from others in a sample.
BD Horizon Brilliant™ polymer conjugates can endow previously dim cells with much brighter fluorescence signals than traditional organic fluorescent dyes or even phycobiliproteins such as PE or APC. Optimization for these bright dyes enables the BD FACSCelesta to identify cell populations with a broader range of receptor density than was previously possible.
Cell Biology Applications
Cell Analysis in a New Light
Flow cytometry can provide rich data to cell biologists working in a wide range of fields, from molecular interaction to systems biology, from pharmacokinetics to cancer biology, from cell signaling to marine biology to biophysics. Use of flow cytometry has blossomed with intensified interest in proteomics, increased use of biomarkers in drug development research, and the spread of high-throughput, cell-based drug screening research.
Detecting and analyzing light scatter allows researchers to measure physical characteristics of cells in suspension, such as cell shape, size, and internal complexity. Adding fluorescent markers allows researchers to interrogate expressed or secreted proteins that reveal cell phenoptype, function, and status. Using a broad landscape of cell function assays, flow cytometry can shed light on a variety of sample types, such as whole blood, cell lines, and yeast. With three lasers and up to 14 optical channels, the BD FACSCelesta can multiplex many of these assays at once on the same sample.
Study Complex Populations at the Single-Cell Level
A major strength of flow cytometry is its ability to study complex populations. Western blots, immunoprecipitation, and PCR-based techniques rely on lysing the entire sample. Their results provide useful data for the population as a whole, but it can be difficult to compare subsets of cells that might behave differently. Other techniques that examine individual cells, such as microscopy, are hard to scale up for quantitative analysis. Flow cytometry can characterize large numbers of individual cells, allowing it to identify, quantify, and characterize different subsets of cells in a heterogeneous population.