Apoptosis

CD4-enriched mouse splenocytes were cultured with anti-CD3/CD28, IL-2, and IL-4

Cell cycle analysis of a population stained for incorporated BrdU and total DNA levels (7-AAD).

The use of VPD450 to correlate cell proliferation with IL-2 production.

Cell cycle analysis of HeLa cells treated with aphidicolin.

Radio frequency dose-dependent apoptosis, necrosis, and cell death monitored by Annexin V - BD Horizon V450.

Flow cytometric analysis of apoptotic and non-apoptotic populations using anti-active caspase-3 antibodies.

In this experiment, Jurkat cells were treated with camptothecin, a potent inhibitor of topoisomerase I and apoptosis inducer.


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