Clinical Discovery Research Reagents
BD Biosciences Clinical Discovery Research Reagents (CDRR) supply clinical researchers with a broad portfolio of tools to advance their clinical discovery.
Our recently expanded portfolio of antibodies and bright fluorescent dyes enables BD to offer a broad selection of standardized kits to advance your clinical discovery research needs.
If you have an idea for markers that you would like to standardize in a research panel, contact BD for the tools and support in your panel design to help accelerate the pace of discovery for your lab.
BD Horizon™ Dri TBNK+CD20
BD Horizon™ Dri TBNK+CD20 is a dried reagent panel comprising the same conjugates that are in the BD Multitest™ 6-color TBNK reagent with the addition of CD20 V450.
Size: 5 Tests
Unit: EA (1 Each)
For a limited time, buy one BD Horizon Dri TBNK+CD20, get one free of charge.Start Ordering
BD Horizon Dri TBNK+CD20 is a dried reagent panel comprising the following conjugates:
Optional Compensation Reagents
- Batch-matched compensation reagent CD4 PE-Cy7
- Batch-matched compensation reagent CD8 APC-Cy7
Store tubes in their original foil pouch at 2-27°C. Do not freeze the reagent or expose it to direct light at any time during storage or incubation with cells. Once the pouch is opened, use the remaining tubes within one month (when stored as directed). Do not use reagents beyond the expiration date indicated on the label. CAUTION: Due to the moisture sensitivity of the reagent, ensure the pouch is immediately and completely resealed after removing a tube. Do not remove the desiccant from the reagent pouch.
Refer to our website or contact your local BD representative for the lyse/no-wash protocol for direct immunofluorescence. Use aseptic blood collected in EDTA (ethylenediamine-tetraacetic acid) blood collection tubes.
- Add 50 μl of well-mixed, anticoagulated whole blood in to the bottom of the air-dried reagent cocktail tube and mix to complete reconstitution by gentle pipetting/vortexing for four seconds. Incubate 15 minutes at room temperature. Protect from exposure to direct light.
- Add 450 μl of 1X BD FACS™ lysing solution to the tube. Vortex to mix. Incubate for 15 minutes in the dark at room temperature.
- The sample is analyzed on a flow cytometer with a setup optimized for antibody panels.
BD Horizon™ Dri Leukocytes
BD Horizon™ Dri Leukocytes are dried lysed whole blood control cells that can be used as positive, negative and procedural controls in a variety of flow cytometric applications. These may include quality control of reagents, procedural controls for immunoassays, routine instrument monitoring and assay design verifications. They can also be used for longitudinal studies and standardizing results across multiple instruments and locations.
Size: 25 tests
Unit: EA (1 each)
Cell count: 2 x 10 6 to 3 x 10 6 cells per tube
Stability: 12 months from manufacturing date, 5 days after reconstitution
Package: The kit includes 1 pouch of 5 tubes and 1 vial of reconstitution buffer.
|CD28||L293||CD66b||G10F5||CD161||DX12||HLA-DR, DP, DQ||TU39|
|CD33||HIM3-4||CD74||M-B741||CD166||3A6||Ms IgG2a IC||G155-178|
|CD41b||HIP2||CD88||D53-1473||CD195||2D7/CCR5||Rt IgG2b IC||A95-1|
BD Horizon Dri Leukocytes should be at stored at 2°C to 8°C. They should not be frozen. Protect the tubes from exposure to humidity during storage and protect from light during and after staining. The cells and reconstitution buffer are stable until the expiration date shown on the labels when stored as directed. Do not use after the expiration date. Due to the moisture sensitivity of BD Horizon Dri Leukocytes, ensure the pouch is immediately and completely resealed after removing a tube. Do not remove the desiccant from the pouch.
Procedure for direct immunostaining of surface markers
- Equilibrate the pouch to room temperature for 5 minutes before opening.
- Remove the desired tubes from pouch and immediately reseal the pouch for long-term storage at 2–8°C.
- Add 500 µL of BD Horizon Dri Leukocyte reconstitution buffer to one tube. Vortex gently to mix.
Note: Each tube contains 5 tests. 100 µL re-suspended cells per test is recommended.
- Add the appropriate volume of staining reagent to each tube, vortex and incubate in the dark for 20 to 30 minutes at room temperature.
- Add 2 mL of 1X PBS wash buffer to the tube. Vortex to mix.
- Centrifuge for 5 minutes at 300–500 g.
- Aspirate the supernatant and re-suspend stained cells in 400 µL of 1% paraformaldehyde (PFA).
- Acquire immediately or store at 2–8°C and acquire within 24 hours of staining.
Visit our website www.bdbiosciences.com/reagents or contact your local BD representative for a comprehensive list of cellular and intracellular markers tested, or specific staining procedures.
This product is made from normal whole human blood cells. The blood used in preparation of this product is tested and found to be negative for human immunodeficiency virus (HIV) type 1 and type 2, and the hepatitis B and hepatitis C viruses. This product contains the preservative ProClin™. All biological specimens are considered biohazards. Handle as if capable of transmitting infection and dispose of with proper precautions in accordance with federal, state and local regulations. Never pipette by mouth. Wear suitable protective clothing, eyewear and gloves.
BD Horizon™ Dri Tumor & Tissue Dissociation Reagent
BD Horizon™ Dri Tumor & Tissue Dissociation Reagent – Gentle, effective dissociation of tissue and tumors for single cell studies.
BD’s new reagent has been designed to maximize cell yields and minimize cell death. The reagent maintains the heterogeneity and diversity of your sample to ensure you see more.
Size: 15 tests/kit (1 test/vial)
Unit: EA (1 each)
Each kit contains 15 single use vials. Each vial of BD Horizon™ Dri Tumor & Tissue Dissociation Reagent contains enough dried reagent to digest from 0.05-1.0 grams of tissue or tumor.
Store dry at 2-8°C. Reagent is stable for 12 months when stored as recommended. See vial label for expiration date.
Reconstitute the contents of one vial in 5 mL DMEM cell culture media to make a 2X stock; keep reconstituted reagent at 37°C until ready to process tissue or tumor sample. Do not use reagent any more than 30 minutes after reconstitution. Manually mince tissue or tumor sample in DMEM cell culture media and combine with 2X Tumor & Tissue Dissociation Reagent stock solution to achieve a 1X concentration. Incubate for 30 min at 37°C with gentle agitation (shaking water bath is best). The recommended sample size is from 0.05-1.0 grams of tissue or tumor. Refer to product insert for a detailed protocol.
For Research Use Only. Not for use in diagnostic or therapeutic procedures.
Cy™ is a trademark of GE Healthcare. Cy™ dyes are subject to proprietary rights of GE Healthcare and Carnegie Mellon University, and are made and sold under license from GE Healthcare only for research and in vitro diagnostic use. Any other use requires a commercial sublicense from GE Healthcare, 800 Centennial Avenue, Piscataway, NJ 08855-1327, USA.
Trademarks are the property of their respective owners.