BD Horizon™ Guided Panel Solution (GPS) tool
Panel design can be a difficult and time-consuming process but is essential to obtaining good data. The BD Horizon™ Guided Panel Solution (GPS) provides a guided workflow for reagent selection based on the principles of panel design. This tool will help you streamline the panel design process and avoid reagent selections that may negatively affect population resolution.
The panel design process has been broken into six steps. At each step, you will be prompted to input information that is pertinent to your panel. The information you will be prompted to enter is information that you likely use when designing panels currently. The tool has information about fluorochromes brightness, instrument configurations and resolution impact, so you do not have to enter that information. The tool takes all of this information into account to provide alerts for reagent selections that may negatively affect population resolution.
The more accurate the inputs are, the more likely the tool will be able to provide relevant outputs. It is also important to note that the tool will not be able to detect every possible reagent selection that may negatively affect resolution, so we cannot guarantee that the panel designed will perform optimally. Additionally, even good panels can have alert messages. This is especially true with higher order panels for which it may be difficult to avoid spectral overlap between reagents. In this case, the tool will allow you to see which populations are most likely to be negatively affected by the reagent selections and trade offs can be assessed.
If you would like to get expert advice prior to running your panel or if you have questions about the data obtained from your panel, our Technical Support team is readily available, just email ResearchApplications@bd.com.
|Panel Iteration 1||Panel Iteration 2||Panel Iteration 3|
|CD127 Alexa Fluor® 647
|BD Horizon GPS notifications of reagent selections that may affect population resolution|
Mismatch of fluorochrome brightness and antigen resolution affects population resolution
Fluorochrome brightness/antigen resolution rules applied, but spectral overlap on co-expressed markers prevents optimal resolution
Principles taken into account and population is well resolved
We welcome feedback on the tool so we can make improvements in later versions.
For Research Use Only. Not for use in diagnostic or therapeutic procedures
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