BD BioCoat™ Matrigel™ Matrix Cellware

BD Matrigel™ Basement Membrane Matrix is a solubilized basement membrane preparation extracted from the Engelbreth-Holm-Swarm (EHS) mouse sarcoma, a tumor rich in ECM proteins. Its major component is laminin, followed by collagen IV, heparan sulfate proteoglycans, entactin and nidogen. BD Matrigel Matrix is effective for the attachment and differentiation of both normal and transformed anchorage-dependent epithelial and other cell types including neurons and oligodendrocytes.

Rat Hepatocytes Cultured in the BD BioCoat™ Hepatocyte Differentiation Environment
Transmission Electron Micrograph of thin sections shows similar intracellular and intercellular structures, indicative of healthy differentiated hepatocytes, in four-week-old cultures.
	Intracellular structures active nucleus (5000x) Numerous mitochondria with calcium deposits; rough ER stacks near cell surfaces and surrounding mitochondria; glycogen stores; golgi, ribosomal rosette, lipid droplets.		Intercellular structures (8600x) Frequent interdigitation of apposing cells; gap and tight junctions; intercellular lumens with microvilli, characteristic of bile canaliculi.

BD BioCoat™ Matrigel™ Matrix Cellware applications include:

• Elicitation of tissue-specific cellular morphology and protein production in epithelial cells
• Differentiation of endothelial, muscle and neuronal cells
• Development of three-dimensional matrix model systems

BD BioCoat™ Matrigel™ Matrix has been used to culture:

• Rat hepatocytes ^{[ 1 ]}
• Primary human hepatocytes ^{[ 2 ]}
• Mouse pituitary gland tissue ^{[ 3 ]}
• Rabbit colonocytes ^{[ 4 ]}
• Human urothelial cells ^{[ 5 ]}
• Osteopontin (OPN) deficient rat vascular smooth muscle cells ^{[ 6 ]}

Source

Quality Control

Matrigel Matrix Cellware:

• Tested for ability to promote neurite outgrowth from chick dorsal root ganglia in the absence of NGF
• Tested and found negative for bacteria and fungi

Matrigel Matrix Cellware - Thin Layer:

• Tested for the ability to promote differentiation (neurite outgrowth) of NG-108 (mouse neuroblastoma/rat glioma) cells.
• Tested and found negative for the presence of bacteria and fungi.

Storage and Stability

• Cellware stable for at least three months at -20°C. Keep frozen until use.
• Thin layer cellware stable for at least three months from date of shipment when stored at 2° to 8°C.

Technical Documents/References

• BD BioCoat Cellware [ PDF ] 

1. Fabrega, A.J., et al., Transplantation 62(12):1866 (1996).
   
2. Krams, S.M., et al., Transplantation 65(5):713 (1998).
   
3. Lee, E.J., et al., Neurosurgery 46(6):1461 (2000).
   
4. Reddy, P.M., et al., Pediatric Research 39(2):287 (1996).
   
5. Solomon, L.Z., et al., J. Lab. & Clin. Medicine 132(4):279 (1998).
   
6. Weintraub, A.S., et al., Lab. Invest. 80(11):1603 (2000).