|
No.
|
Composition |
Storage
& Life |
|
1
|
0.1 M
Potassium Phosphate, pH 7.4
Dilute 200 ml of the 0.5 M Potassium Phosphate, pH 7.4 (Solution
1a)with 800 ml of deionized water. Filter sterilize. |
RT
>1 year |
|
1a
|
0.5 M Potassium
phosphate (KPO4), pH 7.4
Mix 150 mL 0.5 M potassium phosphate, monobasic (Solution No. 1b)
with 700 mL 0.5 M potassium phosphate, dibasic (Solution No. 1c) to
achieve a pH of 7.4. |
RT
>1 year |
|
1b
|
0.5 M Potassium
phosphate, monobasic
Weigh 68.05 grams solid and bring to 1000 mL with deionized water.
Stir to dissolve. |
RT
>1 year |
|
1c
|
0.5 M Potassium
phosphate, dibasic
Weigh 87.09 grams solid and bring to 1000 mL with deionized water.
Stir to dissolve. |
RT
>1 year |
|
2
|
Tris-Mes Buffer, pH 6.8
Add 4.89g (2-[N-Morpholino] ethanesulfonic acid) Hydrate, (Mes),
0.38g Ethylene Glycol - bis (beta-Aminoethylether)-N,N,N',N'-Tetraaceticacid
(EGTA), 0.159g DL-Dithiothreitol (DTT), and 1.86g Potassium Chloride
(KCl) to 350 ml dH2O in a beaker. (Note:The EGTA will
not be in solution at this point). Add Tris-Base until the solution
reaches pH 6.8. Add dH2O to 500 ml, and then add 0.163g
of Sodium Azide. Filter sterilize.
|
4° C or -20° C
6 months |
|
3
|
15mM Zinc
Acetate
Prepare 15mM Zinc Acetate by dissolving 825 mg of Zinc Acetate in
250 ml of de- ionized water. |
4° C
2 weeks |
|
4
|
35 mM
Ammonium Molybdate in 15 mM Zinc Acetate
Dissolve 2.16g of Ammonium Molybdate in 35 ml of 15mM Zinc Acetate.
Bring pH to 5.0 with HCl dilute, and bring solution to 50 ml with
the 15mM Zinc Acetate. Transfer to a polypropylene container wrapped
with aluminum foil. Store at in the dark, for 1 week only. |
4°C,
dark
1 week only |
|
5
|
10% Ascorbic Acid at pH 5.
Add 2 grams of ascorbic acid to 15 mls of dH2O. Bring th
pH to 5.0 with dropwise addition of 40% NaOH, and the final volume
to 20mls with dH2O. |
Freshly prepare for each assay |
|
6
|
10% SDS + Antifoam
A
To 100 mls of 10% SDS add 2-3 drops of Antifoam A. |
RT
6 months |
|
7
|
1mM Verapamil
1mM Verapamil stock solution in dH2O. |
-20°C in small aliquots. |
|
8
|
10mM Sodium Orthovanadate
10mM Sodium Orthovanadate stock solution in dH2O.
|
-20°C in small aliquots. |
|
9
|
50mM MgATP
50mM MgATP stock solution in dH2O. |
-20°C in small aliquots. |
