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BD™ Oxygen Biosensor System
 BD™ Oxygen Biosensor System is an innovative detection system that is adaptable to a wide variety of custom drug discovery applications. As oxygen is consumed, the biosensor fluoresces, providing a signal that can be directly correlated to cell growth or oxygen consumption. BD™ Oxygen Biosensor Systems are ideal for primary and secondary screens measuring viability, toxicity, or antibiotic resistance, as well as for subcellular systems like oxidative enzymes. The proprietary biosensor is available in 96 and 384-well formats.
The BD™ Oxygen Biosensor System is an oxygen sensitive fluorescent compound (tris 1,7-diphenyl-1,10 phenanthroline ruthenium (II) chloride) embedded in a gas permeable and hydrophobic matrix permanently attached to the bottom of a multiwell plate. The concentration of oxygen in the vicinity of the dye is in equilibrium with that in the liquid media. Oxygen quenches the dye in a predictable concentration dependent manner. The amount of fluorescence correlates directly to the rate of oxygen consumption in the well, which in turn can relate to any sort of reaction that can be linked to oxygen consumption.
The unique technology allows homogenous instantaneous detection of oxygen levels, without the need for additional reagents or steps, easy kinetic measurements for growth or drug inhibition assays. The BD™ Oxygen Biosensor System is HTS friendly - the microplate format and easy of detection reduce time consuming and costly steps in the process of drug discovery.
- Guidelines for use of BD™ Oxygen Biosensor System [ PDF ]
- BD™ Oxygen Biosensor System - Frequently Asked Questions [ PDF ]
- Use of the BD™ Oxygen Biosensor System to Determine Doubling Time -- Technical Bulletin 438 [ PDF ]
- BD™ Oxygen Biosensor System Calculating Oxygen Concentration from Fluorescence Data -- Technical Bulletin 443 [ PDF ]
- Monitoring Adherent Cell Proliferations on BD™ Oxygen Biosensor System -- Technical Bulletin 447 [ PDF ]
- BD Oxygen Biosensor System Data Collection and Normalization -- Technical Bulletin 448 [PDF]
- BD Oxygen Biosensor System vs. Exisiting Methods of Measuring Toxicity -- Technical Bulletin 449 [ PDF ]
- Microbial Applications of the BD™ Oxygen Biosensor System [ PDF ]
- BD™ Clontech Matchmaker Biosensor Kit [ PDF ]
- Oxygen Consumption of 3D Cell Constructs as a Nondestructive Predictor of Growth and Differentiation - Poster [ PDF ]
View a listing of published journal articles.
Antibiotics/Microbiology
- A Rapid Method to Determine Disinfectant Efficacy [PDF]
- Development of an automated accelerated homogeneous antibacterial cell-based screen
using BD Oxygen Biosensor Systems and their use in follow-up experiments [PDF]
- High-Throughput Assay System for the Discovery of Anti-Microbial Drugs [PDF]
- High-Throughput for the Discovery of Anti-Bacterial Drugs [PDF]
- Oxygen Biosensor System to Study Sublethally Injured Organisms [PDF]
Cytotoxicity/Metabo-Tox
- A High Throughput, Cell-Based Assay for Metabolic Toxicity [PDF]
- Novel Homogeneous Assay System for Predictive Toxicology Screening [PDF]
Cell Based Applications
- Innate Immune System Signaling: NR8383 Cell Oxidative Burst Measured by Oxygen Consumption [PDF]
- Monitoring the Oxygen Consumption Rates of Cells in Culture [PDF]
- Non-Invasive Monitoring of Oxygen Consumption by 3-Dimensional Tissue Constructs [PDF]
- Novel Plate-Based Cell Viability Assay System [PDF]
- Oxygen Consumption as a Predictor of Growth and Differentiation of MC3T3-E1 Osteoblasts on 3D Biodegradable Scaffolds [PDF]
- Simple, Nondestructive, High Throughput Method to Monitor Oxygen Consumption Rates In Vitro [PDF]
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