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Dispase is a bacillus-derived neutral metalloprotease that is recommended for recovering cells cultured on BD Matrigel™ Basement Membrane Matrix. Dispase will yield a single cell suspension far more gently and effectively than trypsin, collagenase or other proteolytic enzymes; it will not harm cells harvested for subcultivation or bioassays. In addition, Dispase may be used for tissue dissociation. Dispase cleaves fibronectin, collagen IV, and to a lesser extent collagen I, but it does not cleave collagen V or laminin.
Frozen in HBSS, pH 7.4
Bacillus polymyxa-derived metallo protease
36 kD
- Tested for ability to solubilize a 1 mm thick layer of gelled BD Matrigel™ Matrix after two hours at 37°C
- Filtered (0.2 µm membrane) and tested and found negative for bacteria, fungi, and mycoplasma
Recommended concentration - 10 U/cm2 of BD Matrigel™ Matrix (e.g., 100 U per 35 mm dish).
Stable for at least three months at -20°C. Avoid multiple freeze-thaws.
- Dispase - Product Specification Sheet [ PDF ]
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