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The BD Falcon™ TufRol™ Roller Bottle has a one-piece design. While polystyrene does not normally offer high-impact resistance, the BD Falcon™ TufRol™ Roller Bottle is manufactured using a proprietary manufacturing process that provides good impact resistance. This inherent high-strength feature reduces the risk of messy spills and loss of precious contents during accidental mishandling.
Roller bottles are used in both research and manufacturing applications involving the scale-up of mammalian cells for purposes of virus propagation and bioproduct production. BD Falcon™ TufRol™ Roller Bottles are tissue-culture treated, nonpyrogenic and sterilized by gamma irradiation. As with most BD Falcon™ products, our roller bottles are manufactured under a registered ISO 9002 Quality System.
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Multiple Cap Styles and Surface Types
BD Falcon™ TufRol™ Roller Bottles are available with a smooth surface with a growth area of 850 cm2 or a pleated surface with a growth area of 1450 cm2. Easy on/off or vented cap styles are available. The vented cap has an integral hydrophobic 0.2 µm microporous membrane filter vent, allowing consistent gas exchange when the cap is in the closed position.
Automation-Friendly
The one-piece design of BD Falcon Roller Bottles is compatible with manual or automated filling systems. Our roller bottles are supplied with easy on/off polyethylene caps designed to prevent misthreading and to provide a secure seal.
Custom Products
Since many industrial customers have special requirements relative to labeling, lot control, packaging configurations and delivery schedules, we are able to develop custom services and products to suit individual needs. As with all BD Biosciences products, certification is available upon request.
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Yield of BD™ Cell Using Protein-G purification
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First evaluated in roller bottles, BD™ Cell has proven itself a worthy alternative to traditional basal media. While a standard method of culturing antibody, roller bottle production has some limitations: extensive labor and frequent feedings. The initial roller bottle evaluation compared BD Cell™ MAb Medium (basal) with Iscove‘s Modified Dulbecco‘s Medium (IMDM) both supplemented with 10 percent fetal bovine serum (FBS). The cultures were inoculated at a density of 2 x 105 cells/ml. The IMDM was run conventionally with harvesting and feeding performed every two to three days. By contrast, the BD™ Cell culture was run batch fed and incubated untouched for 21 days. Now evaluated in a production environment on more than 300 hybridoma lines, BD Cell‘s average increase of antibody yield 8 to 10 times that of classic IMDM media. Benefits of BD Cell™ MAb Media |
- Manufactured in a Class 100,000 clean room
- One-piece design compatible with manual or automated filling systems
- Proprietary bottle manufacturing process provides greater impact resistance
- Available with smooth or pleated surface, standard or vented cap
- Custom packaging, labeling, and lot control available on request.
- Tissue-culture treated
- Nonpyrogenic
- Sterilized by gamma irradiation
- Integral one-piece design
- Approximate volume 2000 ml
- Manufactured under a registered ISO 9002 Quality System
- BD Falcon™ Cell Culture Products [ PDF ]
- BD Falcon™ TufRol™ Roller Bottles & BD Cell™ MAb Medium [ PDF ]
- BD Falcon™ TufRol™ EZ Roller Bottles--Product Sheet [ PDF ]
Expanded-surface roller bottles should be used on roller racks with the speed set below that used for smooth surface bottles for the same cell type (typically below 1RPM, often as low as 0.1 RPM). |
