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BD Cell™ MAb Media
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BD Cell MAb Media increases yield while dramatically reducing media consumption and labor cost.
Product Description
- BD Cell™ Quantum Yield is a chemically defined non-animal component basal cell culture medium designed to enhance monoclonal antibody production in vitro. This medium will support the growth of a wide variety of myeloma fusion partners and hybridomas including: Sp2/0, NS-1, P3X63Ag9, and FOX-NY as well as other secreting cell lines such as CHO. BD Cell Quantum Yield medium has been found to produce as much as 10 times more antibody when compared to conventional media. Due to its unique formulation, cells can retain viability for longer periods of time and require much less handling than needed with traditional media.
- BD Cell Quantum Yield contains L-glutamine and phenol red. It does not contain pluronic acid or other surfactants.
- BD Cell Quantum Yield is available in 1000 ml bottles; custom packaging is available upon request.
- BD Cell Quantum Yield is a basal medium and must be supplemented in the same way that you supplement your traditional basal medium (i.e., serum or serum-free supplements, antibiotics, etc).
- BD Cell Quantum Yield is a 1X sterile medium with a shelf life of one year if stored at 4ºC in the dark. Do not freeze BD Cell media. Please note that BD Cell media should be more orange-yellow in color than media such as DMEM, IMDM or RPMI 1640.
- BD Cell Quantum Yield medium is a hepes based medium and can be used in either CO2 incubators or non CO2 incubators in a closed system. Always prewarm medium to 37ºC before use.
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Instructions for Use
Production
Some cell lines may adapt readily to BD Cell Quantum Yield medium, but most will require a brief period of adaptation (see protocol attached). Protocols for various culture systems such as roller bottle, hollow fiber and stirred tank are available upon request or can be accessed at: www.bd.com/industrial.
NOTE: Adaptation cultures will not be a true indicator of antibody production potential.
Fusions
BD Cell media have been found to work well in cell fusions thus eliminating the adaptation process for new clones. Normal fusion procedures can be followed with the substitution of BD Cell media for the traditional media used in current fusion procedures.
Downstream Processing
Normal purification procedures such as Protein A or Protein G work well for processing supernatants produced using BD Cell media. High speed centrifugation or filtration procedures such as transgential flow filtration can be used for cellular material removal prior to purification.
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Product Description
- BD Cell Serum-Free is a complete medium designed to enhance monoclonal antibody production in vitro. As with the BD Cell Quantum Yield medium, this serum-free medium will support the growth of a wide variety of myeloma fusion partners and hybridoma including: Sp2/0, NS-1, P3X63Ag9, and FOX-NY as well as other secreting cell lines such as CHO. BD Cell Serum-Free medium has been found to produce as much as 10 times more antibody when compared to conventional serum supplemented media or other commercially available serum-free media. The amount of increased expression will be cell line dependent. Cells will retain viability for longer periods of time and require much less handling than needed with traditional media.
- BD Cell Serum-Free medium contains L-glutamine and phenol red and has a total protein concentration of 1.1 mg/ml. The majority of the protein is comprised of bovine serum albumin. It does not contain pluronic acid or other surfactants.
- BD Cell Serum-Free is available in 1000 ml bottles; custom packaging is available upon request.
- Is a complete medium. However, if you supplement your current medium with special growth factors or lipids, etc., your cells may still need these supplements. A trial period with and without the supplements is recommended.
- BD Cell Serum-Free is a 1X sterile medium with a shelf life of nine months if stored at 4ºC in the dark. Do not freeze BD Cell™ media. Please note that BD Cell media should be more orange-yellow in color than other basal and serum free media.
- BD Cell Serum-Free medium is a hepes based medium and can be used in either CO2 incubators or non CO2 incubators in a closed system. Always prewarm medium to 37ºC before use.
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Instructions for Use
Production
Some cell lines may adapt readily to BD Cell Serum-Free medium, but some may require adaptation first to BD Cell Quantum Yield medium followed by adaptation to the serum-free condition using BD Cell Serum-Free medium (see protocol attached). Protocols for various culture systems such as roller bottle, hollow fiber and stirred tank are available upon request or can be accessed at: www.bd.com/industrial.
NOTE: Adaptation cultures will not be a true indicator of antibody production potential.
Downstream Processing
High speed centrifugation or filtration procedures such as transgential flow filtration can be used for cellular material removal prior to purification. Normal purification procedures such as Protein A or Protein G work well for processing supernatants produced using BD Cell media. The purity of the product should be higher with BD Cell Serum-Free medium as compared to product produced with serum supplemented media. The use of bovine serum albumin in BD Cell Serum-Free medium eliminates the co-purification of bovine IgGs that occurs with serum supplemented media.
Product Description
- BD Cell Animal Component-Free is a complete medium designed to enhance monoclonal antibody production in vitro. It contains no animal components. As with the BD Cell Quantum Yield and Serum-Free media, BD Cell Animal Component-Free medium will support the growth of a wide variety of myeloma fusion partners and hybridomas including: Sp2/0, NS-1, P3X63Ag9, and FOX-NY as well as other secreting cell lines such as CHO. BD Cell Animal Component-Free medium has been found to produce the same dramatic level of antibody expression as that found with the other BD Cell media. The amount of increased expression will be cell line dependent. Cells will retain viability for longer periods of time and require much less handling than is needed with traditional media.
- BD Cell Animal Component-Free contains L-glutamine and is supplemented with 0.3% Select Soytone. It does not contain phenol red or pluronic acid or other surfactants nor does it contain attachment factors which will need to be added if this medium is used with attached dependent cell lines.
- BD Cell Animal Component-Free medium is available in 1000 ml bottles; custom packaging is available upon request.
- BD Cell Animal Component-Free is a complete medium. However, if you supplement your current medium with special growth factors, cholesterol, or lipids, etc., your cells may still need these supplements. A trial period with and without the supplement is recommended.
- BD Cell Animal Component-Free medium is a 1X sterile medium with a shelf life of nine months if stored at 4ºC in the dark. Do not freeze BD Cell media.
- BD Cell Animal Component-Free is a hepes based medium and can be used in either CO2 incubators or non CO2 incubators in a closed system. Always prewarm medium to 37ºC before use.
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Instructions for Use
Production
Some cell lines may adapt readily to BD Cell Animal Component-Free medium while others may require adaptation first to BD Cell Quantum Yield medium followed by adaptation to the animal-free medium (see protocol attached). Protocols for various culture systems such as roller bottle, hollow fiber and stirred tank are available upon request or can be accessed at:www.bd.com/industrial.
Note: Adaptation cultures will not be a true indicator of antibody production potential.
Downstream Processing
High speed centrifugation or filtration procedures such as transgential flow filtration can be used for cellular material removal prior to purification. Purification of products produced using BD Cell Animal Component-Free medium may be greatly simplified due to the low molecular weight of the Select Soytone supplementation. Ammonium sulfate precipitation or diafiltration can be utilized to extract the protein of interest rather than usage of Protein A or Protein G affinity purification.
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- BD Falcon™ Cell Culture Products [ PDF ]
- Trying to get away from Ascites [ PDF ]
- BD Falcon™ TufRol™ Roller Bottles & BD Cell™ MAb Medium [ PDF ]
- Quick Guide for Antibody Production using BD Cell™ MAb Medium in a CELLine™ Device [ PDF ]
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