FITC Mouse Anti-Rat CD61

This SKU will be discontinuing Apr 2024. Suggested alternate SKU is [561909] or for additional support, contact your local applications specialist. Contact Us

BD Pharmingen™ FITC Mouse Anti-Rat CD61

Name: FITC Mouse Anti-Rat CD61
Brand: BD Pharmingen™
SKU: 554952

克隆 F11

(RUO)

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科学资源

商品详情

Brand: BD Pharmingen™

Alternative Name: Integrin β3 chain; Itgb3; Integrin beta-3

Reactivity: Rat (QC Testing)

Isotype: Mouse BALB/c IgG1, κ

Immunogen: Rat (Sprague-Dawley) bone cell suspensions

Application: Flow cytometry (Routinely Tested)

Concentration: 0.5 mg/ml

RRID: AB_395611

Storage Buffer: Aqueous buffered solution containing ≤0.09% sodium azide.

Regulatory Status: RUO

准备和存储

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with FITC under optimum conditions, and unreacted FITC was removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

商品通知

Since applications vary, each investigator should titrate the reagent to obtain optimal results.
Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

554952 Rev. 8

抗体详情

F11

The F11 monoclonal antibody specifically recognizes integrin β3 chain (CD61), which associates with the integrin αv chain (CD51), to form the vitronectin receptor found on endothelial cells, myeloid cells, and osteoclasts, and with the αIIb chain (CD41) on platelets and megakaryocytes. Both receptors mediate adhesion to fibronectin, fibrinogen, vitronectin, thrombospondin, and von Willebrand factor. The F11 antibody reportedly blocks the in vitro attachment of osteoclasts to several ligands. Weak F11 antibody reactivity with human osteoclasts, megakaryocytes, and platelets has also been reported.

554952 Rev. 8

格式详情

FITC

Fluorescein (FITC) is part of the BD blue family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 494-nm and an emission maximum (Em Max) at 518-nm. FITC is designed to be excited by the Blue laser (488-nm) and detected using an optical filter centered near 520 nm (e.g., a 530/30-nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.

格式: FITC

激发源: Blue 488 nm

最多激发源: 494 nm

最多发射源: 518 nm

554952 Rev.8

报价单和参考

研发参考 (3)

Helfrich MH, Nesbitt SA, Dorey EL, Horton MA. Rat osteoclasts adhere to a wide range of RGD (Arg-Gly-Asp) peptide-containing proteins, including the bone sialoproteins and fibronectin, via a beta 3 integrin. J Bone Miner Res. 1992; 7(3):335-343. (Clone-specific: Blocking). 查看参考
Helfrich MH, Nesbitt SA, Horton MA. Integrins on rat osteoclasts: characterization of two monoclonal antibodies (F4 and F11) to rat beta 3. J Bone Miner Res. 1992; 7(3):346-351. (Immunogen: Immunoprecipitation). 查看参考
Kieffer N, Phillips DR. Platelet membrane glycoproteins: functions in cellular interactions. Annu Rev Cell Biol. 1990; 6:329-357. (Biology). 查看参考

554952 Rev. 8

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims. Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

BD Pharmingen™ FITC Mouse Anti-Rat CD61

准备和存储

推荐的实验流程

商品通知

研发参考 (3)