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Rainbow Fluorescent Particles, 3.0-3.4 µm (mid-range FL1 fluorescence)

Sphero™ Rainbow Fluorescent Particles, 3.0-3.4 µm (mid-range FL1 fluorescence)

(RUO)
Rainbow Fluorescent Particles, 3.0-3.4 µm (mid-range FL1 fluorescence)
Rainbow Particle analysis on a BD LSRFortessa™ X-20 flow cytometer.  The panels depict emission signal intensity of the Rainbow Particles for the corresponding fluorochromes.  Top row:  Forward scatter, APC-Cy7 and APC.  Bottom row:  FITC, PE and PerCP-Cy5.5.
Rainbow Particle analysis on a BD LSRFortessa™ X-20 flow cytometer.  The panels depict emission signal intensity of the Rainbow Particles for the corresponding fluorochromes.  Top row:  Forward scatter, APC-Cy7 and APC.  Bottom row:  FITC, PE and PerCP-Cy5.5.
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描述

This product contains a single population of Rainbow Particles that are dyed to a single fluorescent intensity. Every Rainbow Particle contains a mixture of fluorophores that are stably embedded within the particle and can be excited at any wavelength from 365-650 nm, allowing most channels in a flow cytometer to be calibrated using the same set of particles. The Rainbow Particles have emission spectra compatible with many common fluorophores used for immunofluorescent staining by flow cytometric analysis.  Rainbow Fluorescent Particles are provided in a dropper bottle for convenient dispensing and storage.

Sphero™
Aqueous solution containing 0.01%NP40 and ≤ 0.02% sodium azide.
RUO
AB_2869066
Flow cytometry (Tested During Development)


推荐的实验流程

This particle mixture (~10x10^6 particles/mL) is useful for routine calibration of flow cytometers. Before use, resuspend the particles by vortexing. Dilution of 3-5 drops of particles to 1 ml of sheath fluid will provide an adequate number of particles for flow cytometric analysis.

商品通知

  1. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
  2. Cy is a trademark of GE Healthcare.
  3. Please refer to www.bdbiosciences.com/us/s/resources for technical protocols.
556298 Rev. 10

Please refer to Support Documents for Quality Certificates

Global - Refer to manufacturer's instructions for use and related User Manuals and Technical data sheets before using this products as described

Comparisons, where applicable, are made against older BD Technology, manual methods or are general performance claims.  Comparisons are not made against non-BD technologies, unless otherwise noted.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.