BD PureCoat cultureware

Enhanced cell proliferation of MRC-5 cells in growth media containing reduced serum on BD PureCoat carboxyl

A. MRC-5 cells grown in MEM-medium containing 10% serum were trypsinized, washed with serum-free medium and seeded in medium containing 5% fetal bovine serum onto tissue culture (TC)-treated, Competitor C, or BD PureCoat carboxyl plates at 5000 cells/cm2 and cultured for 72 hours. To measure cell proliferation, MTS reagent (Promega) was directly added into culture medium and incubated for 2 hours. Relative absorbance units (mean ± SEM, n=16 wells/surface) of MRC-5 cells grown on BD PureCoat carboxyl 24-well plates show an increase in cell proliferation in reduced-serum versus TC-treated or Competitor C plates.

B. A similar result was also observed on MRC-5 cells grown in 96-well format. Shown are mean increases ± SD (n=7-14 wells/surface) over TC-treated from two independent 96-well plate experiments.

Better freeze-thaw recovery of LnCAP, a prostate cancer cell line on BD PureCoat carboxyl

A. Early passage LnCAP cells cryopreserved in growth medium (RPMI-1640, 10% fetal bovine serum) and 5% DMSO were thawed in a 37°C water bath and immediately seeded onto 96-well black/clear TC-treated, Competitor C, or BD PureCoat carboxyl plates at 16,000 cells/well. After an overnight incubation, cultures were observed and images captured at 100X magnification. Results from a representative experiment show that a greater number of LnCAP cells are evenly attached on BD PureCoat carboxyl versus TC-treated or Competitor C plates.

B. Exhausted media was gently removed, replaced with growth medium containing MTS reagent (Promega) and incubated at 37°C for 2 hours. Relative absorbance units (mean ± SEM, n=14 wells) are highest on BD PureCoat carboxyl compared to those on TC-treated or Competitor C plates.

C. This increase in cell attachment and proliferation was significantly greater (p<0.05) than other surfaces tested (N=3 experiments).

Better freeze-thaw recovery of LnCAP, a prostate cancer cell line, on BD PureCoat carboxyl.

A-B. Early passage LnCAP cells cryopreserved in growth medium (RPMI-1640, L-glutamine, 10% fetal bovine serum) and 10% DMSO were thawed in a 37°C water bath and immediately seeded onto 75 cm² area flasks of TC-treated and BD PureCoat carboxyl at 665,000 cells/flask. After an overnight incubation, growth medium was refreshed and cells allowed to grow. On the 4th day, exhausted medium was aspirated and replenished with 16 ml of fresh growth medium. Cells were allowed to grow for an additional 2-day period and later subjected to trypsinization (0.05% Trypsin-EDTA; 2 ml/flask) for 5 minutes at room temperature. An equal volume of growth media was added to each flask to neutralize trypsin and cells were centrifuged and subjected to cell counting (P1, panel A). Results from a representative experiment show 43% increase in LnCAP cell growth post-thaw attached on BD PureCoat carboxyl vs. TC (n=3 flasks/surface). A 1:10 cell dilution of each flask type was passaged and allowed to grow for an additional 6 days in growth medium. Then, cells were trypsinized and quantified using a hemacytometer. This difference was further enhanced upon passaging (P2, panel B) resulting in 312% on BD PureCoat carboxyl flasks vs. TC.