BD PureCoat cultureware

Applications

• Culture of primary cells, transfected, transformed, and fastidious cell lines
• Culture in standard, serum-free or serum-reduced conditions
• Freeze-thaw recovery of cryopreserved primary cells and cell lines, including division arrested
• Attachment of transfected cells for primary and secondary HTS screening assays
• Transfection of genes into cells
• Alternate to coating cultureware with biological proteins/attachment factors

BD PureCoat™ amine, a positively charged surface, and BD PureCoat carboxyl, a negatively charged surface, provide improved cell attachment, faster cell proliferation, and enhanced recovery post-thaw over standard tissue culture-treated surfaces. These defined surfaces function with a broad range of primary, transfected, transformed, and fastidious cells in standard, serum-free or serum-reduced conditions.

BD PureCoat surfaces support standard cell dissociation techniques, do not interfere with microscopy or imaging analysis, and do not require special steps for cell adaptation.

Defined Animal-Free Cell Culture Surfaces

BD PureCoat amine and carboxyl surfaces are chemically-defined, animal-free enhanced cell culture surfaces that are manufactured using a proprietary surface technology which delivers high lot-to-lot consistency, translating to consistent and reproducible results in cell culture assays. These surfaces also offer a more consistent and defined alternative to coating cultureware with biological proteins and attachment factors, reducing the variability in your culture system and resulting in a more predictable biological outcome.

Improve Attachment, Increase Proliferation, and Enhance Recovery Post-Thaw

BD PureCoat amine and carboxyl surfaces provide improved attachment, increased proliferation, enhanced recovery from freeze-thaw, and improved differentiation for a broad range of cells that exhibit poor attachment properties. This enables you to grow more cells in better defined conditions therefore increasing productivity without sacrificing performance.