Microplates
Surfaces
Choose the Right Surface for your Assay.
Defined and synthetic BD PureCoat™ surface for robust, consistent and reproducible assays.
BD PureCoat microplates have been pre-coated with chemically-defined (synthetic) attachment factors to provide an enhanced surface which is appropriate for a broad range of cell types (primary cells and transformed cell lines) and applications, but is ideal for applications requiring a more defined cell culture environment (serum-free or low serum-containing cultures). BD PureCoat microplates are preferred in a range of screening applications because of its unique chemically-defined surface–a highly controlled environment for optimal cellular growth and more predictable, precise characterization, such as HEK-293 (transfected or not).
BD PureCoat Amine
The novel BD PureCoat Amine, a positively charged surface, provides enhanced cell attachment of primary, transfected, transformed and fastidious cells in standard, serum-free or serum-reduced condition. The results: a robust, consistent and reproducible assay with the benefit of an animal-free and defined surface.
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Enhanced attachment of EcoPack2-293 cells, a viral packaging cell line on BD PureCoat Amine
Cells were seeded onto 384- or 1536-well black/clear BD PureCoat amine, TC-treated, or Competitor C plates at 10,000 cells/well and 2,250 cells/well, respectively, and grown under serum-free conditions for 20-24 hours. The cells were then washed (on a Skatron EMBLA washer) two times with HBSS containing 10 mM Hepes, loaded with calcein AM for 1 hour and read on a PerkinElmer EnVision plate reader. As shown in Figure 1A, pre- and post-wash images indicate that cells remain attached on BD PureCoat amine surfaces and are washed away on other surfaces tested (384-well format). Intra-plate CVs of multiple lots of BD PureCoat amine were < 10% for 384- and 1536-well plate formats, whereas CV’s for TC-treated or Competitor C plates were much greater (Figure 1B) indicating superior reproducibility in cell-based assays on BD PureCoat amine surfaces.
Enhanced attachment of EcoPack2-293 cells, a viral packaging cell line on BD PureCoat Amine
