Microplates
Surfaces
Choose the Right Surface for your Assay.
BD BioCoat™ microplates offered in a variety of surface treatments to provide enhanced cell attachment and growth.
BD BioCoat microplates have been further enhanced (versus TC) with biological coatings of highly purified extracellular matrix (ECM) proteins for the cell culture of more complex cell models, to include transformed cell lines, transfected cells, as well as a variety of primary and stem cells. In complex cell culture environments, BD BioCoat cultureware is the gold standard for dynamic biologic extracellular matrix surfaces designed to mimic in vivo environments and maximize cellular activity, delivering proven results and consistency in a broad range of research applications.
BD BioCoat microplates are coated in a highly controlled, asceptic manufacturing environment to ensure lot-to-lot consistency, assay reproducibility and contamination control.
BD BioCoat Poly-D-Lysine microplates
Poly-D-Lysine (PDL) is a synthetic polymer that enhances cell adhesion and protein absorption by altering surface charges on the culture substrate. As PDL are synthetic molecules, they do not introduce impurities carried by natural polymers. Many transfected cells, but also neuronal cell lines, primary neurons and glial cells have been cultured successfully on PDL.
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BD BioCoat™ PDL 384-well Black/Clear Plates
Competitive Benchmarking of BD BioCoat Poly-D-Lysine (PDL) 384-well Black/Clear Plates
A signal and coefficient of variation (CV) comparison of
BD BioCoat PDL 384-well Black/Clear plates versus respective competitor plates show that BD BioCoat plates exhibit better cell attachment and lower CVs, demonstrating superior performance and consistency. The PDL plates were tested for signal from Calcein AM-labeled BD EcoPack2-293 cell one day after seeding in serum-free medium and washing on a Skatron Washer (Molecular Devices). Intra- and inter-plate percent CVs were measured to ensure even coating. Signal data represents the average of three plates. CV data represents an average of twelve plates, three from four separate experiments.
Competitive Benchmarking of BD BioCoat Poly-D-Lysine (PDL) 384-well Black/Clear Plates
A signal and coefficient of variation (CV) comparison of BD BioCoat PDL 384-well Black/Clear plates versus respective competitor plates show that BD BioCoat plates exhibit better cell attachment and lower CVs, demonstrating superior performance and consistency. The PDL plates were tested for signal from Calcein AM-labeled BD EcoPack2-293 cell one day after seeding in serum-free medium and washing on a Skatron Washer (Molecular Devices). Intra- and inter-plate percent CVs were measured to ensure even coating. Signal data represents the average of three plates. CV data represents an average of twelve plates, three from four separate experiments.
BD BioCoat Collagen I microplates
Collagen I, found in most tissues and organs, is most plentiful in dermis, tendon, and bone. It is an integral part of the framework that holds cells and tissues together and has been recognized as a useful matrix for improving cell culture. In vitro use of collagen can exert effect on the adherence, morphology, growth, migration, and differentiation of a variety of cell types. Typical examples of cells grown on collagen I are endothelial cells (e.g. HUVEC), hepatocytes, muscle cells, PC12 cells, osteoclasts, or transfected HEK-293 cells. Buy Now »
Competitive Benchmarking of BD BioCoat Collagen I 96-well Clear Plates
A signal and CV comparison of BD BioCoat Collagen I 96-well Clear plates versus respective competitor plates show that
BD BioCoat plates exhibit better cell attachment and lower CVs, demonstrating superior performance and consistency. The collagen plates were tested for signal from Calcein AM-labeled HT-1080 cells seeded at 50,000 cells/well one hour after seeding in serum-free medium and hand-washing. Intra- and inter-plate percent CVs were measured to ensure even coating.
Competitive Benchmarking of BD BioCoat Collagen I 96-well Clear Plates
A signal and CV comparison of BD BioCoat Collagen I 96-well Clear plates versus respective competitor plates show that BD BioCoat plates exhibit better cell attachment and lower CVs, demonstrating superior performance and consistency. The collagen plates were tested for signal from Calcein AM-labeled HT-1080 cells seeded at 50,000 cells/well one hour after seeding in serum-free medium and hand-washing. Intra- and inter-plate percent CVs were measured to ensure even coating.
BD BioCoat Poly-L-Lysine cultureware
Poly-L-Lysine (PLL) is a synthetic polymer that enhances cell adhesion and protein absorption by altering surface charges on the culture substrate. As PLL are synthetic molecules, they do not introduce impurities carried by natural polymers. Many transfected cells, but also neuronal cell lines, primary neurons and glial cells have been cultured successfully on BD BioCoat PLL.
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BD BioCoat Poly-D-Lysine/Laminin, Poly-L-Orinthine/Laminin and Laminin/Fibronectin cultureware
BD BioCoat Poly-D-Lysine/Laminin (PDL/LM) and Poly-L-Ornithine/Laminin (PLO/LM) are suitable for culturing many different cell types derived from the Peripheral Nervous System (PNS) and Central Nervous System (PNS), and have been found to promote neural cell attachment and differentiation. BD BioCoat Laminin/Human Fibronectin (LM/HF) provides an in vitro environment that promotes cell attachment and neurite outgrowth. Embryonic rat sympathetic neurons, cerebellar macroneurons, rat astrocytes, and adult human bone marrow stem cells have been successfully cultured on BD BioCoat PDL/LM or PLO/LM. Buy Now »
BD BioCoat Collagen IV cultureware
Type IV Collagen is a ubiquitous component in basement membranes and provides the major structural support for this matrix. When the Collagen IV meshwork is assembled, it provides a scaffold for the assembly of other basement membrane components through interactions with laminin, entactin/nidogen, and heparin sulfate proteoglycan. Among the cells which show enhanced attachment and growth on collagen IV are epithelial cells, human epidermal stem cells, keratinocytes, mouse ES cells, and HT-1080 cells. Buy Now »
BD BioCoat Fibronectin cultureware
Human Fibronectin (HFN) is a widely distributed glycoprotein that is used as a substrate to promote attachment of cells via the well characterized fibronectin RGD motif. The principal function of HFN appears to be in cellular migration during wound healing and development, regulation of cell growth and differentiation, and haemostasis/thrombosis. Among the many cell types which attach, spread, and proliferate on BD BioCoat Fibronectin coated cultureware are smooth muscle cells, endothelial cells, neuroblastoma cells, human myeloma cell lines, lung fibroblast, and mesenchymal stem cells (undifferentiated state). Buy Now »
BD BioCoat Gelatin cultureware
Gelatin provides an attachment and growth substrate for the culture of a variety of cell types. It is also suitable for promoting adhesion of transfected cell types. Gelatin is a heterogeneous mixture of water-soluble proteins derived from the hydrolysis of Collagen. Vascular endothelial cells, HUVEC cells, and embryonic stem cells have been cultured on BD BioCoat Gelatin. Buy Now »
BD BioCoat Laminin cultureware
Laminin (LM), a major component of basement membranes, is a multifunctional glycoprotein that is used as a substrate to culture and maintain differentiated function of a wide variety of cell types. Laminin has been shown to stimulate neurite outgrowth, promote cell attachment, chemotaxis, cell differentiation, and neuronal survival. BD BioCoat Laminin coated cultureware is useful for the culture of various cell types such as neuronal cells, keratinocytes, myoblasts, and breast epithelial cells. Buy Now »
