Bead-Based Immunoassays

Consistent Results with Routine Panels

BD™ CBA Kits provide preconfigured panels for ultimate ease of use. These kits enable multiplex analysis of complex biological samples on a flow cytometer. In contrast to BD CBA Flex Sets, the kits are preconfigured by functional areas of biology (eg, Th1/Th2 or inflammatory cytokines) to measure up to seven analytes simultaneously using capture beads that contain unique amounts of a single red dye. The unique spectral properties of this dye enable analysis of samples on flow cytometers that have a single 488-nm laser or on dual-laser (488-nm or 532-nm and 633-nm) flow cytometers. Each kit comes complete with all of the buffers and reagents necessary to analyze 80 samples.


Consistent packages, pre-optimized, and ready to use

Each assay has been stringently developed for ease of use, rapid data analysis, sensitivity, reproducibility, and quality. Each antibody pair used in the kits is evaluated for dynamic range, sensitivity, and parallel titration curves to native biological samples. In addition, the assay diluent and wash buffers in each kit have been formulated to reduce detrimental effects of serum and plasma proteins on assay performance.


FCAP Array Parameters for BD CBA Kits

Instrument
Reporter Parameter
Clustering Parameters

BD FACSVerse flow cytometer

PE

CBA Red

BD Accuri C6 flow cytometer

FL2

FL4

BD FACSArray™ bioanalyzer

Yellow

Red

BD FACSCanto™ II flow cytometer

PE

APC

BD LSRFortessa™ flow cytometer

PE

APC

BD FACSAria™ III cell sorter

PE

APC

BD FACSCalibur™ flow cytometer (single laser)

FL2

FL3

BD FACSCalibur™ flow cytometer (dual laser)

FL2

FL4