Special Order Products

Special order BD LSRFortessa FSC PMT Option
Special order BD LSRFortessa FSC PMT Option

The BD LSRFortessa™ FSC is optimized for a wide range of biological particles. For applications requiring smaller resolution, a FSC PMT Option is available through the special order program.

Measurement of the antigen expression of human bone marrow cells
Measurement of the antigen expression of human bone marrow cells

Seven-laser excitation of seven fluorochromes is shown, demonstrating the utility of a seven-laser special order BD™ LSR II analyzer.* Each spatially separated beam provides discrete fluorochrome excitation so that virtually no computer compensation is required for complete cell population separation.

Data courtesy of Frederic Preffer (Harvard Medical School, Boston MA). Reference: Preffer F, Dombkowski D. Advances in complex multiparameter flow cytometry: Applications in stem cell research. Cytometry. 2009;76B:295-314.

*The BD LSR product family includes the special order BD LSR II and the BD LSRFortessa. This experiment was run on a BD LSR II configured with the following lasers: 20-mW 355-nm UV, 50-mW 405-nm violet, 20–100 mW 488-nm blue (variable power), 150-mW 532-nm green (variable power), 50-mW 594-nm yellow, 40-mW 638-nm red, and 25-mW 785-nm IR.

Probability state model of human B-cell maturation
Probability state model of human B-cell maturation

Human bone marrow was stained with CD23 Pacific Blue™, CD20 AmCyan, TdT FITC, CD34 PerCP, CD43 PE, CD19 PE-Cy™5.5, CD10 PE-Cy™7, Kappa APC, CD45 APC-Cy7, and Lambda Alexa Fluor® 594. Approximately 10,000 cells were analyzed. The percentage of cells in each state was plotted against the level of antigen expression measured. B-cell precursors are seen to be down-regulating early markers such as CD34 and TdT and up-regulating more mature antigens such as CD20, CD23, and surface immunoglobulin light chain.

Analysis by GemStone™ Software (Verity Software House, Topsham, ME).

Data courtesy of Frederic Preffer (Harvard Medical School, Boston MA). Reference: Preffer F, Dombkowski D. Advances in complex multiparameter flow cytometry: Applications in stem cell research. Cytometry. 2009;76B:295-314.



Cy is a trademark of Amersham Biosciences Corp.

Pacific Blue is a trademark and Alexa Fluor is a registered trademark of Molecular Probes, Inc.