BD FACSDiva™ Software

Single-tube setup for common laser and filter combinations
Single-tube setup for common laser and filter combinations
BD FACSDiva™ software's simplified cytometer setup and daily performance checking uses BD FACSDiva™ CS&T research beads. These uniquely designed, GMP manufactured beads are excited by all currently supplied lasers and emit in the respective detectors for virtually any fluorescent dye.
Setting PMT voltages
Setting PMT voltages
The effect of suboptimal voltage settings on results. Optimal PMT voltage settings are critical for reducing data-classification errors and yielding reliable results. This data demonstrates the effect of varying voltage gains on the estimated percentage of CD4- monocytes.
Example sections of a daily performance report
Example sections of a daily performance report
Daily performance reports from BD FACSDiva software provide up to 30 metrics by which to analyze the performance of the cytometer. A warning flag on the Bright Bead % Robust CV value, for example, might be an indicator of a dirty flow cell.
BD Phosflow monocyte/NK cell activation
BD Phosflow monocyte/NK cell activation
BD FACSDiva software and the BD LSRFortessa™ analyzer combine to provide successful acquisition and analysis of IFN-alpha induced Stat1 activation in subpopulations of EDTA human whole blood using the BD Phosflow™ Monocyte/NK Cell Activation Kit. The kit also includes T-cell marker drop-ins using Brilliant Violet™ 421 and BD Horizon™ PE-CF594 reagents.

Eight-color immunophenotyping on BD FACSCanto II cytometer
Eight-color immunophenotyping on BD FACSCanto II cytometer
Day-to-day reproducibility of 8-color immunophenotyping experiments on the BD FACSCanto™ II cytometer is simplified using BD FACSDiva software and BD FACSDiva CS&T research beads.


For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Brilliant Violet is a trademark of Sirigen Group Ltd.

CF is a trademark of Biotium, Inc.