BD FACSCanto Clinical Software

Simple workflow with automated cytometer setup and compensation

BD FACSCanto™ clinical software automatically sets cytometer detector voltages and creates compensation tables based on a single run with BD FACS™ 7-color setup beads.


A Cytometer Setup Report
 

This standardized approach ensures that fluorescence brightness is correct for stained cells in each detector and improves data integrity. In addition, the software will automatically correct for spectral overlap, also known as spillover, using the setup beads as a reference. Spectral overlap can occur when fluorophores emit light over a range of wavelengths and some of that light "spills over" into detectors other than the primary detector for a given fluorophore. The software uses the newly established spectral overlap values to ensure that stained cells are correctly compensated. The Cytometer Setup Report becomes available after cytometer setup with BD FACS 7-color setup beads is run. The software offers integrated quality control with pass/fail determination, alarms, and error flagging.

A Cytometer Setup Report
A Levey-Jennings report for cytometer setup

Track day-to-day performance with integrated quality control

The Levey-Jennings tool in BD FACSCanto clinical software allows display of cytometer setup results over time in Levey-Jennings statistical process control plots. Problems that might require cytometer maintenance or service can be detected by tracking trends over time. For example, detector voltages should be trended as a key diagnostic to determine if the cytometer is becoming less efficient over time. Less emitted light will reach the detector and a higher PMT voltage will be required for the signal up to the original 7-color setup bead target value, and corrective action can be taken as a result.

A Levey-Jennings report for cytometer setup