BD FACSCanto II

A Powerful Platform for Research Use

For the research lab, the BD FACSCanto™ II system has the versatility to meet demanding research requirements. The system brings BD flow cytometry and multicolor analysis to a new level to help researchers meet complex challenges and advance their research.


BD FACSDiva software for setup, acquisition, and analysis*

BD FACSDiva™ software efficiently controls the setup, acquisition, and analysis of flow cytometry data from the BD FACSCanto II workstation. BD FACSDiva operating software is common across many BD cell analyzers and cell sorters, including BD™ LSR and BD FACSAria™ systems, giving researchers application flexibility.

BD FACSDiva software enables researchers to preview and record data from multiple samples with an automated acquisition process. Acquisition templates, user-defined experiment layouts, and simple compensation procedures also managed by the software further facilitate acquisition.

For analysis, the software includes powerful features such as hierarchical snap-to gating, a variety of plots, and batch analysis. Recorded data can be analyzed by creating plots, gates, population hierarchies, and statistical views on a BD FACSDiva global worksheet. The global worksheet can be used to analyze multiple sample tubes from the same experiment, to save time. User-defined batch analysis and automated capabilities such as gate resizing, pausing between data files, exporting statistics, and printing before proceeding to the next data file are additional BD FACSDiva features.


Cytometer Setup and Tracking

Cytometer Setup and Tracking

BD™ Cytometer Setup and Tracking (CS&T) beads provide, in a single-vial reagent format, automated determination of BD FACSCanto II configuration baselines and monitoring of performance over time using BD FACSDiva software. BD™ CompBeads Compensation Particle Sets are designed to optimize compensation settings in multicolor flow cytometry. Particularly helpful when using tandem dye conjugates, these particle sets simplify establishing compensation corrections for spectral overlap for any combination of fluorochrome-labeled antibodies.


Cytometer Setup and Tracking

BD™ Cytometer Setup and Tracking (CS&T) beads provide, in a single vial reagent format, automated determination of BD FACSCanto II configuration baselines and monitoring of performance over time, using BD FACSDiva software. BD™ CompBeads Compensation Particle Sets are designed to optimize compensation settings in multicolor flow cytometry. Particularly helpful when using tandem dye conjugates, these particle sets simplify establishing compensation corrections for spectral overlap for any combination of fluorochrome-labeled antibodies.

Lysed whole human blood

Eight-color immunophenotyping panel using FITC, PE, PerCP-Cy™5.5, PE-Cy™7, APC, APC-Cy7, AmCyan, and Pacific Blue™ fluorophores.

Research App Lysed blood- Image



Cy™ is a trademark of Amersham Biosciences Corp.

Pacific Blue™ is a trademark of Molecular Probes, Inc.


For Research Use Only. Not for use in diagnostic or therapeutic procedures.