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Bioimaging Systems

Technical Specifications for the BD CARV II™ Confocal Imager

FeatureSpecifications
Confocal scannerNipkow spinning disk (pinholes)
Disk scan rate1000 scans per second
Pinhole diameter70 µm
Spectral transmission360–700 nm
Z-resolution0.5 µm (PSF); 100X PlanApo 1.4NA, 0.8 µm (PSF); 60X PlanApo 1.4NA
Illumination source120-W Hg/metal halide (1200 h) and up to 5 lasers
Internal excitation changerAutomated 8-position wheel (25 mm)
Internal dichroic changerAutomated 5-position wheel (25.7–36 mm)
Internal emission changerAutomated 8-position wheel (25 mm)
Filter sets providedDAPI, E-GFP, Texas Red®; (Semrock Inc., Rochester, New York, BrightLine™ series)
Operation modeAutomated confocal, wide field, bright field
ObservationDirect confocal binocular viewing or camera port
Detector compatibilityCCD camera – Sensicam EM, QE (Cooke); CoolSNAP™ HQ, Cascade 512B (Photometrics); ORCA ER, AG (Hamamatsu), and more
Microscope compatibilityMost infinity corrected fluorescence microscopes with 100% camera port
FRAPAperture control – touch pad or RS232
System controlTouch pad or Computer via RS232 interface
Software driversBD IPLab™; Metamorph®, Image Pro, and others. RS232 command set also available.
Size11(w) x 15.5 (l) x 6(h) inches
27.94 (w) x 39.37 (l) x 15.24(h) cm
Weight14.5 lbs/6.6 kg
Power100–124 V AC/12 V DC

BD CARV II Z-resolution with Olympus objectives

ObjectiveTypeNAMediumMeasured PSF (microns)
40X Water DICPlan Apochromat1.15Water1.2
60X Water DICPlan Apochromat1.2Water1
60X Oil DICPlan Apochromat1.4Oil0.8
100X Oil DICPlan Fluorite1.3Oil0.6
100X Oil DICPlan Apochromat1.4Oil0.5

 For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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