T Cell

BD offers a comprehensive portfolio of reagents and kits to study T cells from different perspectives, using a range of technologies.

Among the methods used for studying T cells, multicolor flow cytometry is preeminent because it enables the characterization of highly complex T-cell subpopulations—both functionally and phenotypically. Complementary technologies such as ELISA, ELISPOT, and bead-based immunoassays also further research in the T-cell area, offering flexibility to meet a range of experimental needs and multiple methods to verify results.

Comparison of technologies for T cell research

The following table lists important characteristics of tools to help you find the ones that meet your particular experimental needs. Certain technologies can reveal specific information about a sample, or might better meet practical needs such as the available instrumentation or sample type. In some cases, researchers can use the combined information from multiple techniques to verify results. Different approaches can paint a detailed picture of the mechanisms contributing to T-cell development.

Tool/Technology Flow Cytometry: Surface Flow Cytometry: Intracellular BD™ Cytometric Bead Array (CBA) ELISPOT ELISA In Vivo Capture Assay
Molecules detected Surface Intracellular and surface Secreted or intracellular Secreted (in situ) Secreted Secreted (in vivo)
Multiparameter Yes Yes Yes No No No
Single cell/cell subset
information
Yes Yes No Frequencies, no subset
information
No No
Antigen-specific Yes Yes Yes Yes Yes Yes
Post-assay viability Yes No Yes, for secreted molecules No Yes Yes
Quantitation of protein Possible* Possible* Yes No Yes Yes
Instrumentation Flow cytometer Flow cytometer Flow cytometer ELISPOT reader Spectrophotometer Spectrophotometer

*With a standard such as BD Quantibrite™ beads

Immunophenotyping: combining surface and intracellular

Using multicolor flow cytometry—and combining surface phenotyping with intracellular staining of cytokines, transcription factors, or signaling molecules—you can identify individual T-cell subsets on the basis of their characteristic marker signature. BD's advanced reagent systems, extensive marker selection, and wealth of support tools help researchers simplify phenotyping and maximize the information obtained from individual samples.

Intracellular cytokines

BD Cytofix/Cytoperm™ reagents and the BD FastImmune™ cytokine system are reagents and protocols optimized to help you use flow cytometry to reproducibly measure cytokines within individual cells.

Intracellular signaling

BD Phosflow™ reagents help you use multicolor flow cytometry to reliably measure the level of phosphorylated proteins involved in T-cell signaling, and combine the data with subset identification.

Secreted cytokines

For detection of secreted cytokines within a sample, BD offers multiple assays. BD Cytometric Bead Array (CBA) technology allows quantitation of multiple soluble cytokines simultaneously, while BD OptEIA™ ELISA reagents are designed for quantitation of single cytokines. BD™ ELISPOT reagents enable determination of the frequency of cytokine-producing cells, and BD™ In Vivo Capture Assays allow quantitation by directly capturing cytokines in vivo.