BD Influx

Intuitive Alignment

The optical system that directs lasers to the interrogation point is easy to use, intuitive, and adaptable. Up to 10 laser paths can be configured. BD is constantly expanding the choice of lasers available for the BD Influx™ system. Check the current technical specifications for the most up-to-date information.


Optics Features - Focus Lens

Final focus lens assembly

The 10 laser paths and 7-pinhole optical collection system support 24 parameters simultaneously and 24 x 24 compensation. Systems with up to 5 lasers support 16 simultaneous parameters and 16 x 16 compensation.

For each laser, light is shaped by individual optics, and final alignment and focusing are achieved using a dedicated lens that steers light to the stream at different angles. For the ten-laser system, the laser paths are collinear through the steering optics and focusing lens, but intersect the stream at seven spatially separated points. Fluorescence is collected through a high NA lens and focused on individual mirrored pinholes, reducing crosstalk between lasers. The modular design of the BD Influx optical system makes it easy to upgrade lasers as research requirements change. In addition, the variety of available lasers is constantly growing to keep pace with the needs of advanced research.

The patented optical design uses a special pinhole camera to image the stream and the pinholes simultaneously, simplifying optical alignment. With the help of the pinhole camera, near optimal alignment can be achieved within seconds without using beads.

Pinhole camera view
Pinhole camera view
The BD Influx system uses a pinhole camera that helps ensure that the fluorescence is in perfect alignment with the detectors. The pinhole camera lets a researcher view the stream at its optical center through the pinhole aperture’s mirrored surface and subsequently align it so that the cells are in focus. When the cells are in focus for the camera, the system is optimally aligned for detecting fluorescence.
Schematic representation of collection lens, pinhole camera, and mirrored pinholes
Schematic representation of collection lens, pinhole camera, and mirrored pinholes
Individual laser positioners
Individual laser positioners
Independent laser beam alignment and video control of laser intercepts and pinholes allow for fast and intuitive alignment of multiple lasers.