BD FACSCalibur

The BD FACSCalibur flow cytometer provides flexibility to support a wide variety of research and clinical applications.


Rare event analysis*

Rare event analysis*

A growing number of flow cytometry–based assays depend on rare-event detection. For example, in the characterization of subpopulations of stem cells, millions of cells are analyzed to achieve a statistically significant sampling of the subset of interest. Advanced applications like this require a flow cytometer capable of high-speed analysis. The BD FACSCalibur™ system features a multi-parameter triggering system that allows a user to accelerate the analysis by electronically eliminating events from on-board processing.

Efficient residual white blood cell enumeration

Efficient residual white blood cell enumeration

The BD Leucocount™ system is the first automated product approved for in vitro diagnostic use for monitoring leucoreduced blood products. Together with BD Trucount™ tubes and BD Leucocount process controls, the BD Leucocount system provides excellent accuracy, precision, and linearity. For high-throughput walkaway capability, the BD FACS™ Loader can also be added to your base BD FACSCalibur system.

Accurate absolute counts with BD Trucount technology

Accurate absolute counts with BD Trucount technology

BD Trucount tubes make single-platform absolute counting easy—no syringes, additional pipetting steps, or need to integrate data from another analyzer. Designed for use with clinical products such as BD Multitest™ and BD Tritest™ lymphocyte subsetting reagents, and the BD Leucocount rWBC assay kit, BD Trucount tubes bring precision to cell counts and improved productivity. For research applications, BD liquid counting beads are also available in the BD™ Cell Viability Kit* for quick estimations of cell cultures or bacterial concentrations.

DNA analysis*

DNA analysis*

The BD FACSCalibur system works together with BD DNA analysis quality control products to eliminate many of the variables associated with ploidy and cell-cycle analysis. The instrument’s optical and fluidic design ensures that high-resolution DNA ploidy and S-phase determinations are easy to achieve. Electronic doublet discrimination, using the area and width of the fluorescence pulse, allows for the removal of artifacts—doublets and aggregates—from the analysis. High-resolution electronics and linearity ensure accurate results. To ensure optimal performance, BD DNA QC products, including fixed cells and fluorescent particles, can be used for setup, verification of the doublet discrimination module, and monitoring daily operation.


 
BD Cytometric Bead Array assays*

BD Cytometric Bead Array assays*

The BD FACSCalibur flow cytometer can be used to acquire data from BD™ Cytometric Bead Array (CBA) multiplexed assays. These assays are powerful, flexible tools that simultaneously quantify multiple cytokine, chemokine, immunoglobulin, or cell signaling targets from a single sample. Choose from preconfigured kits for maximum convenience in screening routine panels of Th1/Th2 or inflammatory cytokines and chemokines, or build your own multiplex assay using the open and configurable BD CBA Flex Set format.

For all of their power, BD CBA assays are fast and remarkably easy to use. Pre-optimized protocols and one-step detection reagents minimize hands-on time and maximize reproducibility.

Immunophenotyping

Immunophenotyping

Characterizing cells at different stages of development through the use of fluorescent-labeled monoclonal antibodies against surface markers is one of the most common applications of flow cytometry. The BD FACSCalibur system provides multicolor capability in an easy-to-use system. Instrument setup is as easy as the push of a button and a click of the mouse. Efficient collection optics, coupled to the flow cell with an optical gel, permit the use of a low-powered, air-cooled argon laser and a red diode laser to ensure the high sensitivity required for analysis of low-density antigens.



For In Vitro Diagnostic use.
Class 1 Laser Product.

* Some options and applications are for Research Use Only. Not for use in diagnostic or therapeutic procedures.